Means and methods for quantitative determination of analyte in liquids

• US 4,627,893 A
• Filed: 03/28/1984
• Issued: 12/09/1986
• Est. Priority Date: 03/28/1984
• Status: Expired due to Fees

• Current Assignee: Dade International, Inc. (Siemens AG)
• Original Assignee: Amdev Incorporated
• Inventors: Cormier, Alan D., Weinberg, Melvin S.
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US06/594,246
• Time in Patent Office: 986 Days
• Field of Search: 204/409, 204/414, 204/411, 204/412, 204/401, 204/402, 204/403, 204/1 T, 73/1 R
• US Class Current: 205/779
• CPC Class Codes: G01N 27/403 Cells and electrode assemblies

Abstract

A testing module is provided having units which can be arranged seriatim to provide narrow through passageways linked to each other for quantitative determination of analytes passing through the units as by electrical testing with electrodes. Auxiliary passageways are preferably used in one unit to allow flushing of a first sample chamber without contamination from a second sample chamber. An electrode holder carries a membrane backed by a gel to aid in electrically linking the membrane to an electrode and to support the membrane in a substantially planar condition in use.

Claims

1. A testing module unit dimensioned and arranged to be used with at least one other module unit in an arrangement for testing an analyte in a liquid,

said module unit having a body and a narrow diameter through passageway,

said passageway having an enlarged chamber intermediate ends of said passageway, said chamber being formed on one side by a membrane of an electrode,

said chamber lying substatially in a plane with a first portion of said passageway opening into an inlet of said chamber at an angle to said plane to cause turbulence substantially confined to said opening while permitting smooth flow beyond said opening whereby said chamber acts as a defuser at its entrance and as a nozzle at an exit thereof,

said chamber inlet having an opposed outlet with said chamber varying in cross section from said opening to exit,

said varying cross section being enlarged toward the center of the chamber and restricted at said exit in a generally diamond shape,

said passageway having a cross-sectional area corresponding to a diameter of from 0.015 inch to 0.05 inch,

said chamber having a maximum cross-sectional area less than ten times the cross-sectional area of said passageway.

2. A testing module unit in accordance with claim 1 and further comprising said chamber defining an area greater than an area defined by said membrane whereby said membrane is wholly encompassed by said chamber.

3. An electrode holder for use in a testing module unit,

said electrode holder comprising an elongated electrode body having a source of electricity connected therethrough to an internal electrode lying within a first chamber therein,

said first chamber being elongated and open to a second chamber underlying a chemically sensitive ion testing membrane,

said second chamber being filled with a gelatinous electrically conductive material and said first chamber being filled with an electrically conductive liquid whereby there is electrical continuity between said internal electrode and said testing membrane.

4. An electrode holder in accordance with claim 3 wherein said second chamber is defined by a second electrode body portion adapted to be mated with said first body portion in sealing engagement therewith to positively bring said gel into contact with said liquid.

5. An electrode holder in accordance with claim 4 wherein said second body portion is in the form of a removable cap having an end surface at which extends said gel.

6. An electrode holder in accordance with claim 3 and further comprising a soft resilient yieldable mount for said membrane defining a planar surface to which said membrane is attached and maintained in substantially planar form,

said mount being adapted to be resiliently compressed to seal said membrane against a cuvette overlying said membrane whereby a sealed chamber can be defined in part by said membrane and said cuvette.

7. An electrode holder in accordance with claim 3 wherein said gel underlies said flexible membrane at an area of said membrane ranging from about 0.0003 square inches to 0.02 square inch.

8. A method of determining the ionic activity of a fluid by passing said fluid in a stream between a first measuring electrode and a second measuring reference electrode,

said first measuring electrode comprising a portion of a sampling chamber, said first measuring electrode having a membrane with a gel of electrically conductive material underlying the membrane and interspersed between it and an internal electrode portion surrounded by a conductive fluid,

and measuring the electromotive force between said reference electrode and said measuring electrode.

9. A method in accordance with the method of claim 8 and further comprising said fluid being in a flowing condition and continuously monitoring said electromotive force.

10. A method in accordance with the method of claim 8 wherein said fluid is static during said measuring.

11. A method in accordance with the method of claim 8 wherein said gel has a viscosity greater than 15,000 centipoise at 25°

C.

12. A method in accordance with the method of claim 8 further comprising said chamber defining a volume greater than passageways leading to and from said chamber with said passageways leading to and from said chamber defining an overall cross section equivalent to a diameter in the range of from 0.015 inch to 0.05 inch.

13. A method in accordance with the method of claim 12 and further comprising said fluid flow in said passageways being directed at angles to said chamber whereby localized turbulence is created at exit of passageway to cleanse said chamber with said chamber acting first as a defuser and second as a nozzle for said fluid in passing through said chamber,

said chamber having a cross section not exceeding ten times the said cross section of said passageways.

14. method in accordance with the method of claim 12 and further comprising said first measuring electrode being in a first test modular unit and said measuring electrode being in a second test modular unit with said passageway extending through said units to permit flow of sample fluid therethrough.

15. A method in accordance with claim 14 and further including a third test modular unit aligned with said first and second units carrying a third measuring electrode to permit measuring another ionic acitivity in said last-mentioned unit.

16. A first testing module unit dimensioned and arranged to be used with at least one other identical module unit in an arrangement for testing an analyte in a liquid,

said module unit having a body, a rear face and a narrow diameter though passageway,

said passageway having an enlarged chamber intermediate ends of the passageway, said chamber being formed on one side by a membrane of an electrode,

said chamber lying substantially in a plane with a first portion of the passageway opened into an inlet of the chamber at an angle to the plane to cause turbulence substantially confined to said opening while permitting smooth flow beyond said opening whereby said chamber acts as a defuser at its entrance and a nozzle at an exit thereof,

said membrane being mounted on an electrode holder,

said electrode holder resiliently urged toward said chamber side,

a second testing modular unit aligned end to end with said first-mentioned modular unit, said second unit having a through passageway mating with said first-mentioned passageway,

said first and second-mentioned units each having outwardly extending side wings and a rear face,

said side wings being mounted seriatim in a housing,

said housing defining a first and second bore passing therethrough and lying adjacent a rear face of said first and second testing modular units respectively,

at least one bore carrying an electrode holder,

said electrode holder being maintained in place by a screw threaded ferrule compressing a sleeve through an O-ring seal engaging a rear face of a unit,

an electrode body of said holder being mounted on said sleeve by a resilient spring engaged by an end cap mounted on said sleeve.

17. A method of cleaning and calibrating a first sampling measuring chamber contained in a modular measuring chamber unit which unit is positioned adjacent a second modular unit containing a reference electrode second chamber,

wherein said units define a passageway between said chambers and through said units,

a second one-way valve passageway intersecting said passageway between said reference electrode second chamber and said sampling measuring chamber, said method comprising

passing a liquid sample through said passageway from said sampling measuring chamber to said reference chamber whereby a measurement of electrical potential can be taken between said chambers while maintaining said second passageway filled with a second fluid other than said sample,

reversing fluid flow between said reference chamber and sampling measuring chamber by passing a cleaning fluid from said reference chamber through said sampling measuring chamber to remove the sample fluid while maintaining said second passageway filled with a fluid,

passing a cleansing fluid flow through said secondary passageway to said sampling measuring chamber without said last-mentioned flow first passing to said reference chamber,

and reversing flow again to pass a calibrant fluid from said sampling chamber to said reference chamber while said secondary passageway maintains a stationary fluid whereby a calibration measurement can be made to measure electrical potential between said sampling chamber and said reference chamber.

18. A method in accordance with the method of claim 17 and further comprising all fluid flow other than the sample fluid flow being of a calibrating liquid.

19. A method in accordance with the method of claim 17 wherein said first-mentioned reversing step is carried out while maintaining said second passageway filled with a stationary fluid.