Positive selection sorting of cells

• US 4,629,687 A
• Filed: 07/29/1982
• Issued: 12/16/1986
• Est. Priority Date: 07/29/1982
• Status: Expired due to Term

• Current Assignee: Board of Trustees E, The Board of Trustees of Michigan State University (Michigan State University)
• Original Assignee: The Board of Trustees of Michigan State University (Michigan State University)
• Inventors: Schindler, Melvin S., Holland, John F.
• Primary Examiner(s): Wiseman, Thomas G.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US06/403,154
• Time in Patent Office: 1,601 Days
• Field of Search: 356/39, 356/73, 422/52, 422/68, 422/22, 436/63, 436/172, 250/458.1, 250/461.1, 250/461.2, 250/222.2, 250/239, 250/304, 250/361 R, 250/461, 250/492.2, 250/564, 250/574, 250/575, 435/4, 435/6, 435/29, 435/30, 435/39, 435/173, 435/241, 435/245, 435/297, 435/310, 435/800, 435/808, 435/311, 435/291, 435/261, 209/3.1, 209/3.3, 209/44.1, 209/540, 209/541, 209/552, 209/578, 209/579, 209/644, 209/906, 209/932, 209/934
• US Class Current: 435/4
• CPC Class Codes: B07C 5/342 according to optical properties, e.g. colour according to radiation transmittivity B07C5/3416
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties

Abstract

A method and apparatus for positive selection of viable cells based upon differing chemical or physical properties or dynamic processes using a focussed radiant energy beam, such as a laser beam (11) to kill unwanted cells is described. The apparatus includes a microscope (14) with a photomultiplier (27) which is used to selectively detect light from certain cells produced by an attenuated laser beam irradiating the cells for identifying the cells to be killed or saved. Based upon identification, selected cells to be killed are exposed to the unattenuated laser beam.

Claims

1. A method for automatically sorting anchorage dependent cells by selective destruction of unwanted cells in situ comprising:

(a) culturing a heterogeneous population of anchorage dependent cells on a support surface;

(b) identifying wanted and unwanted cells by;

(i) focussing a focussed radiant energy beam at a non-lethal energy level upon each cell, thereby eliciting a distinguishable response;

(ii) monitoring the response with a discriminating means;

(iii) determining the wanted from unwanted cells with a control means;

(iv) selectively altering the intensity of a focussed radiant energy beam to a lethal energy level thereby destroying unwanted cells;

(v) directing the movement of said lethal and said non-lethal beam means in relationship to said surface with an automatic coordinating means so as to impact all cells in a motion coordinated with said discriminating means and automatically adjusting said lethal means to destroy said unwanted cells.

2. An apparatus for automatically sorting anchorage dependent cells by selective destruction of unwanted cells in situ comprising:

(a) a cell support surface;

(b) a first focussed radiant energy beam selectively directed in a path intersecting said cell support surface, said first beam and said surface relatively moveable with respect to each other to access said beam to all cells attached to said surface;

(c) discriminating means which monitors a response of said cells to said first beam at a non-lethal energy level;

(d) a second focussed radiant energy beam adjusted selectively to alter the intensity of said second focussed radiant energy beam at a lethal energy level thereby destroying said unwanted cells;

(e) control means which determines wanted from unwanted cells; and

(f) automatic coordinating means selectively directing the movement of said beams in relationship to said surface so as to impact all cells with said beams in a motion coordinated with said discriminating means and automatically adjusting said beams to destroy said unwanted cells.

3. An apparatus in accord with the claim 2 in which said beams are laser beams.

4. An apparatus in accord with claim 2 in which the cell support surface is moveable in respect to said radiant energy beams and wherein said cell support is operably attached to environmental support means for life support of said cells on said support surface, and wherein movement and environment is controlled by said automatic coordinating means.

5. An apparatus in accord with claim 2, said discriminating means being a shielded photomultiplier tube observing said cells and sensing the response of said cells to said first focussed radiant energy beam to coordinate said response with the function of said automatic coordinating means.

6. An apparatus in accord with claim 5 in which said automatic coordinating means is computer directed.

7. The apparatus of claim 6 in which said automatic coordinating means is connected controllably to cell environment and flushing means in preservation of life support for said cells on said cell surface and in avoidance of residue from destroyed cells contamining remaining wanted cells.

8. An apparatus for automatically sorting anchorage dependent cells by selective destruction of unwanted cells in situ, the combination comprising:

a cell support surface having connections for nutritional, thermal and chemical adjustment of cell environment;

a focussed radiant energy beam having at least two energy intensities and directed in a path intersecting said cell support surface, said beam and said surface relatively moveable with respect to each other to access said beam to all cells attached to said surface;

cell discriminating means in an aligned relation with said beam and intersecting said cell surface in congruence with said beam and being correlated to said beam to first select a non-lethal energy intensity for recognition of wanted and unwanted cells, said discriminating means providing criteria for the selection of a second lethal energy intensity for said beam;

adjusting means providing the feedback control connection responsive to criteria provided by said discriminating means and selective of the energy intensity of said beam; and

automatic coordinating means in control of movement of said beam in relation to said cell support surface in traversing the entire of said surface, automatically responsive to recognition by said discriminating means and shifting of said beam from non-lethal to lethal intensity by manipulation of said adjusting means, and selectively sustaining the environment of said cells on said support surface in preservation of cell life support conditions and the flushing of debris from said support surface during and after lethal exposure of unwanted cells on said support surface to said beam at lethal intensity.