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Optical sensor for fluorescence measurements

  • US 4,632,807 A
  • Filed: 12/21/1983
  • Issued: 12/30/1986
  • Est. Priority Date: 12/28/1982
  • Status: Expired due to Fees
First Claim
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1. An optical sensor apparatus which has an operating mode and a non-operating mode and when in its operating mode can measure the partial pressure of a particular gas in a liquid or gaseous sample medium and when in its operating mode comprisesa sample chamber for containing the liquid or gaseous sample medium which contains a particular gas whose partial pressure is to be measured, said sample chamber having first and second opposite sides,a separate reference chamber for containing a reference medium, said reference chamber having first and second opposite sides,a light source for radiating excitation light toward the first side of each of said sample chamber and said reference chamber,a unitary excitation filter positioned between said light source and the first side of both said sample chamber and said reference chamber, said unitary excitation filter covering the first side of both said sample chamber and said reference chamber and functioning to filter the excitation light from said light source which passes to each of said sample chamber and said reference chamber,a single fluorescent sensor element positioned to cover the second sides of both said sample chamber and said reference chamber, said single fluorescent sensor element including a first portion and a second portion and a first side and a second side, the first side of said first portion facing said sample chamber and the first side of said second portion facing said reference chamber, and the second sides of both first and second portions facing away from said sample chamber and said measuring chamber respectively,first and second separate emission filter means located on the second side of said single fluorescent sensor element, said first and second emission filter means having first sides which face said single fluorescent sensor element and second sides facing away therefrom, the first side of said first emission filter means facing the second side of said first portion of said single fluorescent sensor element and the first side of said second emission filter means facing the second side second portion of said single fluorescent sensor element, andfirst and second photodetectors respectively located on the second sides of said first and second emission filter means, said optical sensor apparatus operating such that rays of excitation light from said light source will pass through different areas of said unitary excitation filter, then through each of said sample chamber and said reference chamber, then into said first and second portions of said single fluorescent sensor element, thereby generating fluorescent radiation which is quenched in said first and second portions by the respective sample medium and reference medium, the fluoresent radiation generated in said first and second portions of said single fluorescent sensor element respectively then passing through said first and second emission filter means and respectively to said first and second photodetectors.

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