Optical sensor for fluorescence measurements

• US 4,632,807 A
• Filed: 12/21/1983
• Issued: 12/30/1986
• Est. Priority Date: 12/28/1982
• Status: Expired due to Fees

• Current Assignee: AVL AG
• Original Assignee: AVL AG
• Inventors: Marsoner, Hermann
• Primary Examiner(s): Evans, F. L.
• Assistant Examiner(s): Harringa, Joel L.
• Application Number:
  US06/563,931
• Time in Patent Office: 1,105 Days
• Field of Search: 250/373, 250/461.1, 250/458.1, 356/306, 356/317, 356/318, 356/417, 422/83, 422/86, 422/87, 422/91, 422/68, 436/172
• US Class Current: 422/82.08
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/643 non-biological material

Abstract

In an optical sensor used mainly for determining the partial pressure of O₂ or CO₂ in respiratory gas includes a sample measuring chamber through which sample medium to be analyzed flows or into which it is fed, a reference measuring chamber containing a reference gas or a reference liquid of constant composition, a light source, a unitary excitation filter positioned between the light source and first sides of both the sample measuring chamber and the reference measuring chamber, a single fluorescent sensor element on the second sides of both the sample measuring chamber and the reference measuring chamber, and first and second detectors for detecting the fluorescence radiation from the fluorescent sensor element respectively based on the light passing thereto from the sample measuring chamber and the reference measuring chamber.

Claims

1. An optical sensor apparatus which has an operating mode and a non-operating mode and when in its operating mode can measure the partial pressure of a particular gas in a liquid or gaseous sample medium and when in its operating mode comprises

a sample chamber for containing the liquid or gaseous sample medium which contains a particular gas whose partial pressure is to be measured, said sample chamber having first and second opposite sides,

a separate reference chamber for containing a reference medium, said reference chamber having first and second opposite sides,

a light source for radiating excitation light toward the first side of each of said sample chamber and said reference chamber,

a unitary excitation filter positioned between said light source and the first side of both said sample chamber and said reference chamber, said unitary excitation filter covering the first side of both said sample chamber and said reference chamber and functioning to filter the excitation light from said light source which passes to each of said sample chamber and said reference chamber,

a single fluorescent sensor element positioned to cover the second sides of both said sample chamber and said reference chamber, said single fluorescent sensor element including a first portion and a second portion and a first side and a second side, the first side of said first portion facing said sample chamber and the first side of said second portion facing said reference chamber, and the second sides of both first and second portions facing away from said sample chamber and said measuring chamber respectively,

first and second separate emission filter means located on the second side of said single fluorescent sensor element, said first and second emission filter means having first sides which face said single fluorescent sensor element and second sides facing away therefrom, the first side of said first emission filter means facing the second side of said first portion of said single fluorescent sensor element and the first side of said second emission filter means facing the second side second portion of said single fluorescent sensor element, and

first and second photodetectors respectively located on the second sides of said first and second emission filter means, said optical sensor apparatus operating such that rays of excitation light from said light source will pass through different areas of said unitary excitation filter, then through each of said sample chamber and said reference chamber, then into said first and second portions of said single fluorescent sensor element, thereby generating fluorescent radiation which is quenched in said first and second portions by the respective sample medium and reference medium, the fluoresent radiation generated in said first and second portions of said single fluorescent sensor element respectively then passing through said first and second emission filter means and respectively to said first and second photodetectors.

2. An optical sensor apparatus according to claim 1, wherein said light source is symmetrically positioned with respect to said sample chamber and said reference chamber.

3. An optical sensor apparatus according to claim 1, including a temperature control device for maintaining the temperature of said fluorescent sensor element constant.

4. An optical sensor apparatus according to claim 1, additionally comprising a sensor housing and a thermostat, add wherein said sample chamber and said reference chamber are maintained at the same constant temperature by said thermostat.

5. An optical sensor apparatus according to claim 1, further comprising a sensor housing and a light housing, wherein said sensor housing contains said single fluorescent sensor element, said first and second emission filter means, and said first and second photodetectors, and wherein said light housing contains said light source, said unitary excitation filter, said sample chamber and said reference chamber, wherein said sensor housing is pivotally attached to said light housing such that said sensor element and said light housing can be moved together to cause said optical sensor apparatus to be in its operating mode or moved apart to cause said optical sensor apparatus to be in its non-operating mode, and including means for fixedly connecting said sensor housing to said light housing when they are moved together.

6. An optical sensor apparatus according to claim 5, wherein said single fluorescent sensor element provides the second side of both said sample chamber and said reference chamber when said sensor housing and said light housing are moved together.

7. An optical sensor apparatus according to claim 1, wherein said single fluorescent sensor element further comprises a thin coat of polymer material containing an oxygen indicator which is applied to a transparent rigid carrier.