Adsorbent and process for preparing the same

• US 4,637,994 A
• Filed: 05/28/1985
• Issued: 01/20/1987
• Est. Priority Date: 12/02/1982
• Status: Expired due to Term

• Current Assignee: Kanegafuchi Kagaku Kogyo Kabushiki Kaisha
• Original Assignee: Kanegafuchi Kagaku Kogyo Kabushiki Kaisha
• Inventors: Hayashi, Tsuneo, Tani, Nobutaka
• Primary Examiner(s): SHINE, WILLIAM
• Application Number:
  US06/737,880
• Time in Patent Office: 602 Days
• Field of Search: 502/400, 502/401, 502/402, 502/403, 502/404
• US Class Current: 502/404
• CPC Class Codes: A61M 1/3679 by absorption A61M1/3675 takes precedence
• CPC Class Codes: A61M 2202/0057 retained by adsorption
• CPC Class Codes: A61M 2202/0413 Blood
• CPC Class Codes: A61M 2202/0415 Plasma
• CPC Class Codes: A61M 2202/046 Low-density lipoprotein
• CPC Class Codes: A61M 2230/005 Parameter used as control input for the apparatus
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/327 Polymers obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens

Abstract

An adsorbent for removing low and/or very low density lipoprotein from body fluid in extracorporeal circulation treatment, which comprises a water-insoluble porous hard gel with exclusion limit of 10⁶ to 10⁹ daltons on which a sulfated compound is immobilized by a covalent linkage.

Claims

1. An adsorbent for removing low and/or very low density lipoprotein from body fluid in extracorporeal circulation treatment, which comprises a water-insoluble porous hard gel with exclusion limit of 10⁶ to 10⁹ daltons on which a sulfated compound is immobilized by a covalent linkage;

said sulfated compound being a compound obtained by sulfation of a hydroxy-containing compound.

2. The adsorbent of claim 1, wherein said water-insoluble porous hard gel is a water-insoluble porous polymer hard gel.

3. The adsorbent of claim 2, wherein said water-insoluble porous polymer hard gel is a porous cellulose gel.

4. The adsorbent of claim 1, wherein said water-insoluble porous hard gel is a porous inorganic hard gel.

5. The adsorbent of claim 4, wherein said water-insoluble inorganic hard gel is a member selected from the group consisting of porous glass, porous silica gel and porous alumina.

6. The adsorbent of claim 1, wherein the sulfated compound is a sulfated carbohydrate.

7. The adsorbent of claim 6, wherein the sulfated carbohydrate is a sulfated saccharide.

8. The adsorbent of claim 7, wherein the sulfated saccharide is a sulfated polysaccharide.

9. The adsorbent of claim 8, wherein the sulfated polysaccharide is a member selected from the group consisting of heparin, dextran sulfate, condroitin sulfate and salts thereof.

10. The adsorbent of claim 9, wherein the dextran sulfate, a salt thereof or a mixture of the dextran sulfate and the salt has an intrinsic viscosity of not more than 0.12 dl/g and a sulfer content of not less than 15% by weight.

11. The adsorbent of claim 1, wherein the sulfated compound is a sulfated polyhydric alcohol.

12. The adsorbent of claim 1, wherein the exclusion limit is 10⁶ to 10⁸ daltons.

13. The adsorbent of claim 1, wherein said sulfated compound is immobilized in an amount of 0.02 to 100 mg/ml of bed volume.

14. The adsorbent of claim 13, wherein the sulfated compound is immobilized in an amount of not less than 0.2 mg/ml of bed volume.

15. A process of preparing an adsorbent for removing low and/or very low density lipoprotein from body fluid in extracorporeal circulation treatment comprising a water-insoluble porous hard gel with exclusion limit of 10⁶ to 10⁹ daltons on which a sulfated compound is immobilized, wherein said water-insoluble porous hard gel is reacted with epichlorhydrin or a polyoxirane compound to introduce epoxy groups on to the gel, and then the resulting epoxy-activated gel is reacted with the sulfated compound;

said sulfated compound being a compound obtained by sulfation of a hydroxy-containing compound.

16. The process of claim 15, wherein said water-insoluble hard gel is a water-insoluble porous polymer hard gel.

17. The process of claim 16, wherein said water-insoluble porous polymer hard gel is a porous cellulose gel.

18. The process of claim 15, wherein said sulfated compound is dextran sulfate, a salt thereof or a mixture of the dextran sulfate and the salt;

said dextran sulfate, the salt thereof or the mixture of the dextran sulfate and the salt being reacted with the epoxy-activated gel in a concentration of not less than 3% by weight based on the weight of the whole reaction system excluding the dry weight of the porous hard gel.

19. The process of claim 18, wherein the porous hard gel is a porous cellulose gel.