Whole blood clotting timer
First Claim
1. A method for determining the clotting time for a whole blood sample comprising inserting the whole blood sample into a first, radially inner compartment of a disposable cuvette in a centrifuge, inserting a blood clotting activator into a second, radially outer compartment of that cuvette which is separated from the first compartment by a ridge in the floor of the cuvette, actuating the centrifuge to cause the whole blood sample to flow radially outwardly over the ridge and to mix with the blood clotting activator in a second compartment and simultaneously starting timing means running, continuing the operation of the centrifuge to sediment the blood cells against a radially outer wall of the second compartment, periodically measuring the optical density of a part of the mixture in the second compartment radially inwardly of said wall thereof which part of the mixture is freed from blood cells, stopping the timing means when the optical density or the time rate of change of the optical density of the mixture reaches a predetermined level, thereafter stopping the centrifuge and indicating the time interval between the starting and stopping of the timing means.
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Accused Products
Abstract
A method and device for determining the clotting time for a sample of whole blood. A sample of whole blood is placed in a cuvette (12) which is centrifuged in a centrifuge (11) to mix the blood sample with a coagulant in the cuvette (12) and simultaneously spin out the cells in the blood. The optical density of a part of the sample which is cleared of cells by the centrifugation is monitored using a light source (14) and a light detector (15) and the time measured for optical density indicative of clotting to be reached by a timer (25).
19 Citations
1 Claim
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1. A method for determining the clotting time for a whole blood sample comprising inserting the whole blood sample into a first, radially inner compartment of a disposable cuvette in a centrifuge, inserting a blood clotting activator into a second, radially outer compartment of that cuvette which is separated from the first compartment by a ridge in the floor of the cuvette, actuating the centrifuge to cause the whole blood sample to flow radially outwardly over the ridge and to mix with the blood clotting activator in a second compartment and simultaneously starting timing means running, continuing the operation of the centrifuge to sediment the blood cells against a radially outer wall of the second compartment, periodically measuring the optical density of a part of the mixture in the second compartment radially inwardly of said wall thereof which part of the mixture is freed from blood cells, stopping the timing means when the optical density or the time rate of change of the optical density of the mixture reaches a predetermined level, thereafter stopping the centrifuge and indicating the time interval between the starting and stopping of the timing means.
Specification
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- Resources
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Current AssigneeUnisearch Limited (University Of New South Wales)
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Original AssigneeUnisearch Limited (University Of New South Wales)
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InventorsBertram, Christopher D., Milthorpe, Bruce K., Farrell, Peter C.
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Primary Examiner(s)NOT, DEFINED
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Application NumberUS06/514,828Time in Patent Office1,316 DaysField of Search422/72, 422/73, 436/69, 436/45, 436/34, 436/805US Class Current436/34CPC Class CodesG01N 33/4905 Determining clotting time o...Y10T 436/111666 Utilizing a centrifuge or c...
