Composition and method for ethanol determination
First Claim
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1. A method for determining the level of ethanol in a fluid or air comprising exposing absorbent material to said fluid or air, the absorbent material being impregnated with a composition comprising:
- (a) An indicator solution comprising orthotolidine, a yellow dye, ethanol and tris(hydroxymethy)-aminomethane/sodium phosphate buffer solution and(b) an enzyme solution comprising dextran, sodium chloride, peroxidase, alcohol oxidase, semicarbazide hydrochloride and a tris(hydroxymethyl)-aminomethane/sodium phosphate buffer solution said dextran and sodium chloride being present in amounts sufficient to stabilize the alcohol oxidase and the semicarbazide hydrchloride being present in an amount sufficient to inhibit said alcohol oxidase.
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Abstract
A novel composition and method is disclosed for detecting or quantitatively determining the level of ethanol. This is a colorimetric method and analysis can be done by the visual method. The composition comprises an enzyme alcohol oxidase, a peroxide detecting system consisting of peroxidase and chemicals and chemicals to stabilize alcohol oxidase.
36 Citations
8 Claims
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1. A method for determining the level of ethanol in a fluid or air comprising exposing absorbent material to said fluid or air, the absorbent material being impregnated with a composition comprising:
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(a) An indicator solution comprising orthotolidine, a yellow dye, ethanol and tris(hydroxymethy)-aminomethane/sodium phosphate buffer solution and (b) an enzyme solution comprising dextran, sodium chloride, peroxidase, alcohol oxidase, semicarbazide hydrochloride and a tris(hydroxymethyl)-aminomethane/sodium phosphate buffer solution said dextran and sodium chloride being present in amounts sufficient to stabilize the alcohol oxidase and the semicarbazide hydrchloride being present in an amount sufficient to inhibit said alcohol oxidase. - View Dependent Claims (2, 3, 4)
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- 5. A composition of making ethanol detection strips comprising dextran, sodium chloride, peroxidase, alcohol oxidase, semicarbazide, a buffer solution and a hydrogen peroxide detecting reagent said dextran and sodium chloride being present in amounts sufficient to stabilize the alcohol oxidase and the semicarbazide hydrochloride being present in an amount sufficient to inhibit said alcohol oxidase.
Specification