Culturing and emplacement of differentiated cells in vivo

• US 4,645,669 A
• Filed: 10/04/1982
• Issued: 02/24/1987
• Est. Priority Date: 10/04/1982
• Status: Expired due to Term

• Current Assignee: Albert Einstein College of Medicine (Yeshiva University)
• Original Assignee: Albert Einstein College of Medicine of Yeshiva University
• Inventors: Reid, Lola C. M.
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US06/432,552
• Time in Patent Office: 1,604 Days
• Field of Search: 424/177, 424/95, 435/1, 435/240
• US Class Current: 424/520
• CPC Class Codes: A61F 2/02 Prostheses implantable into the body closure means for urethra or rectum or for artificial body openings therefor A61F2/0004
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: C12N 2533/90 Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A method and culture solution are disclosed which enable in vivo emplacement of differentiated cells derived from in vitro culturing of differentiated cells, with significant retention of their differentiated character. In an alternative embodiment, in vivo culturing of differentiated cells is contemplated. Through the use of extracellular matrix fibers, specifically derived from connective tissue, as culture substrates, the method also discloses the isolation of the connective tissue fibers and their preparation as a culture substrate. This method provides significantly higher survival and attachment rates, and often significantly improved growth properties for in vivo or in vitro cultures of differentiated cells, especially epithelial, over the current methods for culturing these cells. This method also significantly enables certain differentiated cells to retain much of their normal enzymatic activities, and furthermore, this method enables certain differentiated cells to retain to a high degree, their ability to secrete substances, such as hormones.

Claims

1. A method for providing an improved in vivo site for promoting the growth of site-specific differentiated cells, comprising providing site-specific connective tissue-derived fibers comprising collagens, non-collagenous proteins and carbohydrates as a substrate for in vivo cell culture of site-specific differentiated cells at the site.

2. The method of claim 1 in which the site-specific differentiated cells are mammalian cells.

3. The method of claim 1 in which the site-specific differentiated cells are epithelial cells.

4. The method of claim 1 in which the site-specific differentiated cells are body cells that are normally in contact with basal lamina material.

5. The method of claim 35 in which the site-specific connective tissue-derived fibers are isolated from organ tissue.

6. A method for the in vivo culturing of site-specific differentiated cells which comprises selecting an in vivo site to be provided with site-specific differentiated cells, preparing said site for the receipt of culture medium, and disposing a quantity of culture medium comprising site-specific connective tissue-derived fibers comprising collagens, non-collagenous proteins and carbohydrates as a substrate for the in situ culturing of site-specific differentiated cells at said in vivo site, and applying differentiated cells to the culture medium whereby in situ growth of site-specific differentiated cells takes place.

7. The method of claim 6 in which the resultant in situ growth of the culture of site-specific differentiated cells in vivo is bound to cells originally at the in vivo site.

8. The method of claim 6 in which the site-specific differentiated cells are mammalian cells.

9. The method of claim 6 in which the site-specific differentiated cells are epithelial cells.

10. The method of claim 6 in which the site-specific differentiated cells are body cells that are normally in contact with basal lamina material.

11. The method of claim 6 in which the site-specific connective tissue-derived fibers are isolated from organ tissue.

12. A method for the emplacement at an in vivo site of site-specific differentiated cells which comprises providing an initial supply of site-specific differentiated cells, disposing said initial supply in an in vitro culture medium comprising site-specific collagens, non-collagenous proteins and carbohydrates as a substrate for the in vitro cell culture, whereby in vitro culturing of said site-specific differentiated cells takes place and the cell culture remains substantially differentiated, and transferring and transplanting the resulting growth of the site-specific differentiated cell culture to said in vivo site to be provided with site-specific differentiated cells, so that the supply of differentiated cells at said in vivo site is increased.

13. The method of claim 12, wherein the temperature at which the in vitro culturing method is conducted is optimal for the cellular growth in vitro.

14. The method of claim 12, wherein the site-specific differentiated cells are mammalian.

15. The method of claim 12, wherein the site-specific differentiated cells are non-mammalian cells.

16. The method of claim 12, wherein the site-specified differentiated cells are epithelial cells.

17. The method of claim 12, wherein the site-specific differentiated cells are body cells that are normally in contact with basal lamina material.

18. The method of claim 12, wherein the site-specific connective tissue-derived fibers are isolated from organ tissue.

19. The method of claim 12, further comprising placing the site-specific connective tissue-derived fibers onto culture solid-state supports in vitro, and allowing said fibers to cover said solid-state supports.

20. The method of claim 19, further comprising sterilizing said solid-state supports in vitro, after adding the site-specific connective tissue-derived fibers.

21. The method of claim 20, further comprising adding the site-specific differentiated cells to the tissue culture plates in vitro after sterilization.

22. The method of claim 20, wherein the site-specific connective tissue-derived fibers attach to the solid-state support in vitro.