Volume independent test device

• US 4,647,430 A
• Filed: 06/20/1985
• Issued: 03/03/1987
• Est. Priority Date: 06/20/1985
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Zweig, Stephen E.
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Johnston, Jill
• Application Number:
  US06/746,767
• Time in Patent Office: 621 Days
• Field of Search: 422/56, 422/57, 422/58, 435/805, 436/169, 436/170
• US Class Current: 422/424
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

A volume independent test device is formed in which the reagent matrix is covered by a water-impermeable coating or membrane. At least three openings extend through the coating or membrane material, penetrating into the underlying reagent matrix. These openings permit liquid to pass through the coating or membrane material and into the reagent matrix material until the reagent matrix becomes saturated.

Claims

1. A volume independent test device comprising a reagent matrix covered by an adhering layer having means defining at least three openings extending through said adhering layer and penetrating into the underlying reagent matrix which permit liquid to pass through said adhering layer into the reagent matrix material until the reagent matrix becomes saturated, said adhering layer being positioned to cover all of the liquid contacting surface of said reagent matrix and being constructed of a material which is impermeable or semipermeable to liquid.

3. The test device of claim 1 in which the number of openings range from about 10 to about 50.

4. The test device of claim 1 in which the percentage of surface area occupied by said openings ranges from about 0.01 to about 5 percent.

5. The test device of any of claims 1 to 4 in which the diameter of the openings is from about 0.01 to about 1 mm.

6. The test device of any of claims 1 to 4 in which the openings extend from about 10 percent to about 100 percent into the underlying reagent matrix.

7. The test device of any of claims 1 to 4 in which the adhering layer has a thickness of from about 0.005 to about 2 mm.

10. The test device of any of claims 1 to 4, in which said means defining at least three openings permits only 20 to 25 microliters of liquid to pass through the adhering layer to the underlying reagent matrix.

2. The test device of caim 1 in which the number of openings range from about 3 to about 300.

8. A volume independent test device comprising at least one reagent matrix composed of a bibibulous material covered by a membrane containing means defining openings which occupy from about 0.01 percent to about 5 percent of the total area on the top surface of said membrane and in which said openings extend through said membrane and a distance of 10 to 100 percent into said bibulous material, said membrane being positioned to cover all of the liquid contacting surface of said bibulous material and said membrane being impermeable or semipermeable to liquid.

9. A test device comprising a reagent matrix covered by an adhering layer having means defining openings which occupy from about 0.01 percent to about 5 percent of the total surface area of the top surface of said adhering layer and in which the openings extend through said adhering layer and a distance of 10 to 100 percent into said reagent matrix thereby obtaining a test device capable of constant sample loading per unit area of reagent matrix, said adhering layer being positioned to cover all of the liquid contacting surface of said reagent matrix and being constructed of a material which is impermeable or semipermeable to liquid.