Method of encapsulating biomaterial in bead polymers
First Claim
Patent Images
1. A method of immobilizing viable animal cells, plant cells, bacteria, algae or fungi with retained ability of growth by encapsulation in polymer beads, which comprises:
- (a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen;
(b) dispersing said aqueous solution in a non-toxic water-insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and
(c) allowing said agar, agarose or fibrinogen to gel to form polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi either by cooling or by enzymatic action under conditions such that the growth ability of said cells is unaffected.
0 Assignments
0 Petitions
Accused Products
Abstract
Viable biomaterial such as animal cells, plant cells, bacteria, algae or fungi are immobilized with retained ability of growth by encapsulation in polymer beads. Encapsulation is carried out by adding the biomaterial to an aqueous solution of a polymer such as agar, agarose, carrageenan, chitosan, gelatin, collagen or fibrinogen, dispersing the solution in a water-insoluble dispersion medium such as soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil or phthalic acid dibutylester and allowing the polymer to gel.
-
Citations
6 Claims
-
1. A method of immobilizing viable animal cells, plant cells, bacteria, algae or fungi with retained ability of growth by encapsulation in polymer beads, which comprises:
-
(a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen; (b) dispersing said aqueous solution in a non-toxic water-insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and (c) allowing said agar, agarose or fibrinogen to gel to form polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi either by cooling or by enzymatic action under conditions such that the growth ability of said cells is unaffected. - View Dependent Claims (2, 3, 5, 6)
-
-
4. Immobilized viable animal cells, plant cells, bacteria, algae or fungi with a retained ability of growth, and being encapsulated in polymer beads of agar, agarose or fibrinogen, and being produced by:
-
(a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen; (b) dispersing said aqueous solution in a non-toxic water insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and (c) allowing said agar, agarose or fibrinogen to gel to form the polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi such that the growth ability of said cells is unaffected.
-
Specification