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Method of encapsulating biomaterial in bead polymers

  • US 4,647,536 A
  • Filed: 11/08/1983
  • Issued: 03/03/1987
  • Est. Priority Date: 03/08/1982
  • Status: Expired due to Term
First Claim
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1. A method of immobilizing viable animal cells, plant cells, bacteria, algae or fungi with retained ability of growth by encapsulation in polymer beads, which comprises:

  • (a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen;

    (b) dispersing said aqueous solution in a non-toxic water-insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and

    (c) allowing said agar, agarose or fibrinogen to gel to form polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi either by cooling or by enzymatic action under conditions such that the growth ability of said cells is unaffected.

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