Method of encapsulating biomaterial in bead polymers

• US 4,647,536 A
• Filed: 11/08/1983
• Issued: 03/03/1987
• Est. Priority Date: 03/08/1982
• Status: Expired due to Term

• Current Assignee: Kjell Nilsson, Klaus Mosbach
• Original Assignee: Kjell Nilsson, Klaus Mosbach
• Inventors: Nilsson, Kjell, Mosbach, Klaus
• Primary Examiner(s): Naff, David M.
• Assistant Examiner(s): Foley, Shawn P.
• Application Number:
  US06/552,044
• Time in Patent Office: 1,211 Days
• Field of Search: 264/4.1, 264/4.3, 424/32, 424/34-38, 424/93, 424/94, 435/177, 435/182, 435/178, 514/2
• US Class Current: 435/177
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: C12N 11/04 entrapped within the carrier, e.g. gel or hollow fibres
• CPC Class Codes: Y10S 424/07 Microporous membranes

Abstract

Viable biomaterial such as animal cells, plant cells, bacteria, algae or fungi are immobilized with retained ability of growth by encapsulation in polymer beads. Encapsulation is carried out by adding the biomaterial to an aqueous solution of a polymer such as agar, agarose, carrageenan, chitosan, gelatin, collagen or fibrinogen, dispersing the solution in a water-insoluble dispersion medium such as soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil or phthalic acid dibutylester and allowing the polymer to gel.

Claims

1. A method of immobilizing viable animal cells, plant cells, bacteria, algae or fungi with retained ability of growth by encapsulation in polymer beads, which comprises:

(a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen;

(b) dispersing said aqueous solution in a non-toxic water-insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and

(c) allowing said agar, agarose or fibrinogen to gel to form polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi either by cooling or by enzymatic action under conditions such that the growth ability of said cells is unaffected.

2. The method as claimed in claim 1, wherein said gelation is conducted so as to obtain beads having a granular size of 0.05-3 mm.

3. The method as claimed in claim 2, wherein said gelation is conducted so as to obtain beads having a granular size of 0.1-1.0 mm.

5. The method as claimed in claim 1, wherein said retained growth ability of said cells is such that after the encapsulation of said viable animal cells, plant cells, bacteria, algae or fungi, a relative respiration in the range of 82-100% is exhibited.

6. The immobilized cells as claimed in claim 5, wherein the retained growth ability of said cells is such that after the encapsulation of said viable animal cells, plant cells, bacteria, algae or fungi, a relative respiration in the range of 82-100% is exhibited.

4. Immobilized viable animal cells, plant cells, bacteria, algae or fungi with a retained ability of growth, and being encapsulated in polymer beads of agar, agarose or fibrinogen, and being produced by:

(a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen;

(b) dispersing said aqueous solution in a non-toxic water insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and

(c) allowing said agar, agarose or fibrinogen to gel to form the polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi such that the growth ability of said cells is unaffected.