Dispensing apparatus for storing, draining and dispensing beads

• US 4,648,529 A
• Filed: 06/12/1985
• Issued: 03/10/1987
• Est. Priority Date: 06/12/1985
• Status: Expired due to Fees

• Current Assignee: Cetus Corporation (Bayer AG)
• Original Assignee: Cetus Corporation (Bayer AG)
• Inventors: Hanamoto, Mark S., Blakemore, Judith I., Williams, Fred G.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US06/743,798
• Time in Patent Office: 636 Days
• Field of Search: 221/1, 221/96, 221/97, 221/98, 221/133, 221/135, 221/197, 221/268, 221/276, 221/286, 221/287, 221/288, 221/289, 222/1, 222/130, 222/131, 215/6, 220/20, 220/20.5, 210/803, 210/800, 210/514, 43/54.1, 43/55, 43/56
• US Class Current: 221/1
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B65D 81/22 in moist conditions or immersed in liquids

Abstract

An apparatus and process for storing beads in a liquid medium and for draining and dispensing said beads essentially in the absence of said liquid medium is described. In one embodiment, the apparatus is designed so that the liquid medium in which the beads are stored can be captured in a separate compartment from the bead-containing compartment and the beads can be delivered without the liquid medium when the apparatus in inverted. This apparatus is designed so that the liquid medium will join with the beads for storage in the bead-containing compartment when the apparatus is upright. In another embodiment, the apparatus includes a bottle with a removable, threaded inner container which is used to drain the liquid medium from the beads prior to attachment to a separate bead dispenser.

Claims

1. An apparatus for storing beads in a liquid medium and dispensing said beads essentially in the absence of said liquid medium comprising a threaded bottle, a container for the beads which is positioned in the interior of said bottle and contains at least one drainage port and a threaded open neck, a primary closure cap equipped with threads for the bottle, and a separate dispensing unit threaded to fit said container.

2. The apparatus of claim 1 wherein the liquid medium is a buffer and the beads are conjugated to an immunoassay reagent.

3. The apparatus of claim 2 wherein the reagent is an antibody or antigen.

4. An apparatus for storing beads in a liquid medium and dispensing said beads essentially in the absence of said liquid medium, comprising a bottle and a primary closure cap, said bottle comprising a bottom, a neck and an interior, and further comprising (a) a bottomless funnel positioned in the interior of said bottle, said funnel having a wide bottom portion and a narrow neck portion and attached only at the neck of said bottle, which funnel is capable of storing the beads in a liquid medium when the bottle is in an upright position, is capable of draining the liquid medium from the beads into the bottle exterior of the funnel when the bottle is placed on one side or inverted with the cap removed, and contains an outlet hole at its neck sufficiently large to pass the beads to the exterior of said apparatus, and (b) a dispenser housing fitted within said neck of said bottle, which housing contains an escapement mechanism to control dispensing of the beads from the funnel.

5. The apparatus of claim 4 wherein said escapement mechanism is provided with a means for allowing only one bead at a time to pass through said outlet hole of said funnel.

6. The apparatus of claim 4 wherein said funnel contains at least one drainage port which is positioned to allow the liquid medium to drain from the beads when the apparatus is positioned on one side or inverted with the primary cap removed.

7. The apparatus of claim 6 wherein said funnel contains a plurality of drainage ports distributed over the entire funnel.

8. The apparatus of claim 4 wherein said liquid medium is a buffer, said beads are conjugated to an immunoassay reagent, and said apparatus is composed of plastic.

9. The apparatus of claim 8 wherein said reagent is an antibody or antigen.

10. The apparatus of claim 4 wherein said funnel is permanently attached to the bottle.

11. The apparatus of claim 4 wherein said funnel is removable from the bottle.

12. The apparatus of claim 4, wherein a sealing member is fitted between the neck of said bottle and said funnel, said sealing member being provided with reservoirs to trap additional liquid and lend additional support to said funnel.

13. A process for dispensing, essentially in the absence of a liquid medium, beads which are stored in a container containing liquid medium and fitted in the interior of a bottle, said bottle having a bottom, a neck, and an interior, and being provided with a primary closure cap at its neck, said container being capable of storing the beads in a liquid medium when the bottle is in an upright position, capable of draining the liquid from the beads into the bottle exterior of the container when the bottle is placed on one side or inverted for dispensing the beads, said container being provided with an outlet hole sufficiently large to pass the beads to the exterior of the bottle and said container further being provided with an escapement mechanism for controlling dispensing of the beads from said bottle, said process comprising inverting said bottle without its cap to drain the beads and engaging said escapement mechanism.

14. The process of claim 13 wherein said liquid medium is a buffer and said beads are conjugated to an immunoassay reagent.

15. The process of claim 14 wherein said reagent is an antibody or an antigen.

16. The process of claim 13 wherein the container is a bottomless funnel positioned in the interior of said bottle with its widest portion at the bottom of said bottle and its narrowest portion at the neck of said bottle and attached only at the neck of said bottle, wherein said escapement mechanism is fitted in a dispenser housing positioned within the neck of the bottle to dispense the beads from the bottle.

17. The process of claim 16 wherein said escapement mechanism is configured such that only one bead at a time passes through said outlet hole of said funnel.

18. The process of claim 16 wherein said funnel contains at least one drainage port which is positioned to allow liquid to drain from the beads when the container is positioned on one side or inverted.

19. The process of claim 18 wherein said funnel contains a plurality of drainage ports distributed over the entire funnel.

20. The process of claim 16 wherein said liquid medium is a buffer, said beads are conjugated to an immunoassay reagent, and said bottle and cap are composed of plastic.

21. The process of claim 17 further comprising after dispensing said beads the steps of capping and uprighting or positioning on one side said bottle so as to store the remaining beads in said liquid medium.

22. The process of claim 21 wherein additional liquid medium is added to the container to restore liquid levels.

23. The process of claim 16 wherein said dispenser housing contains a sealing member on said funnel with reservoirs to trap additional liquid and lend additional structural support to said funnel.

24. A process for storing, draining and dispensing beads, comprising:

providing an apparatus comprising a threaded bottle, a container for the beads which is positioned in the interior of said bottle and contains at least one drainage port and a threaded open neck, a primary closure cap equipped with threads for the bottle, and a separate dispensing unit threaded to fit said container;

filling the container of the apparatus with beads and adding sufficient liquid medium to the bottle to submerge all of said beads in the liquid medium and attaching the cap thereto for storage;

removing the cap;

positioning the container so as to drain liquid medium from the beads;

attaching said dispensing unit to the container; and

dispensing the beads.

25. The process of claim 24 wherein the liquid medium is a buffer and the beads are conjugated to an immunoassay reagent.

26. The process of claim 25 wherein the reagent is an antibody or antigen.

27. A process for storing, draining and dispensing beads, comprising:

providing an apparatus for storing beads in a liquid medium and dispensing said beads essentially in the absence of said liquid medium, comprising a bottle and a primary closure cap, said bottle having a bottom, a neck, and an interior, and further being provided with a bottomless funnel positioned in the interior of said bottle, said funnel having a wide bottom portion and a narrow neck portion and attached only at the neck of said bottle, which funnel is capable of storing the beads in a liquid medium when the bottle is in an upright position, is capable of draining the liquid medium from the beads into the bottle exterior of the funnel when the bottle is placed on one side or inverted with the cap removed, and contains an outlet hole at its neck sufficiently large to pass the beads to the exterior of said apparatus, and a dispenser housing fitted within said neck, which housing contains an escapement mechanism to control dispensing of the beads from the funnel;

filling the funnel of the apparatus with beads and adding sufficient liquid medium to the bottle to submerge all of said beads in the liquid medium and attaching the cap thereto for storage;

removing the cap;

positioning the bottle so as to drain liquid medium from the funnel; and

positioning the bottle to dispense the beads and engaging said escapement mechanism to release the beads, said escapement mechanism being configured such that only one bead at a time passes through said outlet hole of said funnel.

28. The process of claim 27 wherein after dispensing or draining said beads the cap is replaced and the apparatus is uprighted to store the beads or positioned on one side to drain or store the beads.

29. The process of claim 28 wherein after dispensing said beads but before replacing the cap additional liquid medium is added to the apparatus.

30. The process of claim 27 wherein the liquid medium is a buffer and the beads are conjugated to an immunoassay reagent.

31. The process of claim 30 wherein the reagent is an antibody or antigen.