Recombinant bacterial plasmids containing the coding sequences of insulin genes

• US 4,652,525 A
• Filed: 06/28/1983
• Issued: 03/24/1987
• Est. Priority Date: 04/19/1978
• Status: Expired due to Term

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Shine, John, Chirgwin, John, Pictet, Raymond, Ullrich, Axel, Rutter, William J., Goodman, Howard M.
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/508,651
• Time in Patent Office: 1,365 Days
• Field of Search: 435/172.3, 435/68, 435/70, 435/71, 435/253, 435/317
• US Class Current: 435/252.33
• CPC Class Codes: C07K 14/62 Insulins
• CPC Class Codes: C12N 15/00 Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00
• CPC Class Codes: Y10S 435/849 Escherichia coli

Abstract

A recombinant procaryotic microorganism containing the gene coding for insulin.

Claims

1. A recombinant plasmid replicable in procaryotic host containing within its nucleotide sequence a subsequence having the structure of the reverse transcript of an mRNA of a vertebrate, which mRNA encodes insulin.

6. A plasmid according to claim 1 comprising a plasmid containing at least one genetic determinant of col E1.

2. A recombinant procaryotic microorganism modified to contain a nucleotide sequence having the structure of the reverse transcript of an mRNA of a vertebrate, which mRNA encodes insulin.

4. A microorganism according to claim 2 wherein the vertebrate is a mammal.

5. A microorganism according to claim 2 wherein the vertebrate is a human.

7. A microorganism according to claim 2 comprising a strain of Escherichia coli.

3. The bacterium Escherichia coli which has been modified to contain a nucleotide sequence having the structure of and transcribed from the rat gene for insulin.