Instrumentless quantitative analysis system

• US 4,654,310 A
• Filed: 01/10/1984
• Issued: 03/31/1987
• Est. Priority Date: 01/10/1984
• Status: Expired due to Term

• Current Assignee: Uy-Vu Ly
• Original Assignee: Uy-Vu Ly
• Inventors: Ly, Uy-Vu
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Delahunty, C. M.
• Application Number:
  US06/569,891
• Time in Patent Office: 1,176 Days
• Field of Search: 435/805, 436/810, 436/169, 436/174, 436/95, 436/164, 422/56, 422/57, 422/58, 422/61
• US Class Current: 436/164
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

A method for generating a series of digital responses to the presence of a given concentration of analyte in a test sample is described. The responses are obtained by providing varying levels of components of a competing reaction with constant levels of components of a control reaction. The pattern of digital responses permits a direct reading of the level of analyte in the test sample. Analytes may be either substrates for enzyme reactions, or may be, themselves, enzymes.

Claims

1. A solid support matrix suitable for determining the concentration of an analyte in a sample, which matrix comprises:

a support divided into a plurality of test portions, wherein each one of said test portions contains first reaction and second reaction components,

said first reaction components including at least a first catalyst capable of reacting with said analyte in a first reaction the concentration of said first catalyst being the same in each one of said individual test portions,

said second reaction component including at least a second catalyst capable of reacting with said analyte in a second reaction in competition with said first catalyst, the concentration of said second catalyst being different in each one of said test portions,

wherein either said first reaction or second reaction is an indicator reaction which produces a detectable result, and the other of said first and second reactions is a non-indicator reaction which does not produce a detectable result,

whereby when a sample containing said analyte is added to each of said test portions an array of test portions having different results is obtained, such that in test portions where the ratio of indicator reaction catalyst to non-indicator reaction catalyst is high, the result of the indicator reaction is detectable, and in test portions wherein said ratio is low, the result of said indicator reaction is not detectable,

whereby the concentration of analyte in said sample is determinable by observing the point at which changeover from detectable to non-detectable indicator reaction occurs.

2. The matrix of claim 1 wherein the first reaction components are the components for the indicator reaction so that in test portions where the ratio of second to first catalyst is low, said indicator reaction is detectable and in test portions where the ratio is high, said indicator reaction is non-detectable.

3. The matrix of claim 1 wherein the second reaction components are the components for the indicator reaction so that in test portions where the ratio of second to first catalyst is high, said indicator reaction is detectable and in test portions where the ratio is low, said indicator reaction is non-detectable.

4. A solid support matrix suitable for determining the concentration of a catalyst-analyte in a sample, which matrix comprises:

a support divided into a plurality of test portions, wherein each one of said test portions contains first reaction and second reaction components,

said first reaction components including at least a substrate capable of reacting with said catalyst-analyte in a first reaction, the concentration of said substrate being the same in each one of said individual test portions,

said second reaction component including at least a second catalyst capable of reacting with said substrate in a second reaction in competition with said catalyst-analyte, the concentration of said second catalyst being different in each one of said individual test portions,

wherein either said first reaction or second reaction is an indicator reaction which produces a detectable result, and the other of said first and second reactions is a non-indicator reaction which does not produce a detectable result,

whereby when a sample containing said catalyst-analyte is added to each of said test portions an array of test portions having different results is obtained, such that in test portions where the ratio of indicator reaction catalyst to non-indicator reaction catalyst is high, the result of the indicator reaction is detectable, and in test portions wherein said ratio is low, the result of said indicator reaction is not detectable,

whereby the concentration of catalyst-analyte in said sample is determinable by observing the point at which changeover from detectable to non-detectable indicator reaction occurs.

5. The matrix of claim 4 wherein the second reaction components are components for the non-indicator reaction so that in test portions where the ratio of second catalyst to catalyst-analyte is low, said indicator reaction is detectable and in test portions where the ratio is high, said indicator reaction is non-detectable.

6. The matrix of claim 4 wherein the second reaction components are components for the indicator reaction so that in test portions where the ratio of second catalyst to catalyst-analyte is high, said indicator reaction is detectable and in test portions where the ratio is low, said indicator reaction is non-detectable.

7. A method for determining the concentration of an analyte in a sample comprising:

dividing a sample into a plurality of individual test portions;

combining each one of said plurality of test portions with first reaction and second reaction components,

said first reaction components including at least a first catalyst capable of reacting with said analyte in a first reaction, the concentration of said first catalyst being the same in each one of said individual test portions,

said second reaction components including at least a second catalyst capable of reacting with said analyte in a second reaction in competition with said first catalyst, the concentration of said second catalyst being different in each one of said individual test portions,

wherein either said first reaction or second reaction is an indicator reaction which produces a detectable result, and the other of said first and second reactions is a non-indicator reaction which does not produce a detectable result,

whereby an array of test portions having different results is obtained, such that in test portions where the ratio of indicator reaction catalyst to non-indicator reaction catalyst is high, the result of the indicator reaction is detectable, and in test portions wherein said ratio is low, the result of said indicator reaction is not detectable,

whereby the concentration of analyte in said sample is determinable by observing the point at which changeover from detectable to non-detectable indicator reaction occurs.

8. The method of claim 7 wherein the first reaction is the indicator reaction so that in test portions where the ratio of second to first catalyst is low, said indicator reaction is detectable and in test portions where the ratio is high, said indicator reaction is non-detectable.

9. The method of claim 7 wherein the second reaction is the indicator reaction so that in test portions where the ratio of second to first catalyst is high, said indicator reaction is detectable and in test portions where the ratio is low, said indicator reaction is non-detectable.

10. The method of claim wherein said first and second reactions are conducted on a solid support matrix.

11. A method for determining the concentration of a catalyst-analyte in a sample comprising:

dividing a sample into a plurality of individual test portions;

combining each one of said plurality of test portions with first reaction and second reaction components,

said first reaction components including at least a substrate capable of reacting with said catalyst-analyte in a first reaction, the concentration of said catalyst-analyte being the same in each one of said indiviual test portions,

said second reaction component including at least a second catalyst capable of reacting with said substrate in a second reaction in competition with said catalyst-analyte, the concentration of said second catalyst being different in each one of said individual test portions,

wherein either said first reaction or second reaction is an indicator reaction which produces a detectable result, and the other of said first and second reactions is a non-indicator reaction which does not produce a detectable result,

whereby an array of test portions having different results is obtained, such that in test portions where the ratio of indicator reaction catalyst to non-indicator reaction catalyst is high, the result of the indicator reaction is detectable, and in test portions wherein said ratio is low, the result of said indicator reaction is not detectable,

whereby the concentration of catalyst-analyte in said sample is determinable by observing the point at which changeover from detectable to non-detectable indicator reaction occurs.

12. The method of claim 11 wherein the first reaction is the indicator reaction so that in test portions where the ratio of second catalyst to catalyst-analyte is low, said indicator reaction is detectable and in test portions where the ratio is high, said indicator reaction is non-detectable.

13. The method of claim 11 wherein the second reaction is the indicator reaction so that in test portions where the ratio of second catalyst to first catalyst-analyte is high, said indicator reaction is detectable and in test portions where the ratio is low, said indicator reaction is non-detectable.

14. The method of claim 11 wherein said first and second reactions are conducted on a solid support matrix.