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Apparatus and method for sensing species, substances and substrates using oxidase

  • US 4,655,880 A
  • Filed: 08/01/1983
  • Issued: 04/07/1987
  • Est. Priority Date: 08/01/1983
  • Status: Expired due to Fees
First Claim
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1. In combination, a pair of potentiostat operating electrode systems for measuring in an electrolyte the concentration of a substance, species or substrate reactive, in the presence of an enzyme, with an electrochemical constituent of the electrolyte, said electrode systems each including a respective working electrode in association with a counter electrode and a reference electrode, and circuit means for energizing said electrodes while maintaining a substantially constant potential between each working electrode and the associated reference electrode with the reference electrode at equilibrium with the electrolyte, the working electrode of one electrode system interfacing not in the presence of said enzyme with the electrolyte, such that the current flowing between the working and counter electrodes of said one electrode system will be functionally related to balanced but reverse reactions between the electrochemical constituent and the electrolyte at such electrodes, and the working electrode of the other electrode system interfacing in the presence of said enzyme with the electrolyte, such that current flowing between the working and counter electrode of said one system is functionally related to an imbalance between such reverse reactions at the working electrode and counter electrode resulting from consumption of electro-chemical constituent by reaction with the substance, species or substrate.

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