Foto/phoresis apparatus

• US 4,657,655 A
• Filed: 01/30/1986
• Issued: 04/14/1987
• Est. Priority Date: 01/30/1986
• Status: Expired due to Term

• Current Assignee: Fotodyne Incorporated
• Original Assignee: Fotodyne Incorporated
• Inventors: Prieto, Thomas E., Gette, William R., Smoot, Jeffrey B., Vitek, Richard K.
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/824,133
• Time in Patent Office: 439 Days
• Field of Search: 204/299 R, 204/182.3
• US Class Current: 204/612
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

An apparatus for electrophoretically separating, visualizing and photographing DNA fragments in agarose gels, the apparatus including an electrophoresis chamber havng a platform and an electrode located on each side of the platform, a transilluminator having a UV light source, a frame defining a viewing opening over said light source, and a UV filtering cover for enclosing said viewing opening, a tray having movable gates for defining an enclosure for the agarose gel in one position and for locating the tray on the platform in the chamber in the other position, and a camera assembly adapted to be mounted on the frame in the transilluminator for providing 1:1 magnification photographs of the DNA fragment migration patterns.

Claims

1. An apparatus for separating and identifying DNA fragments in agarose gels comprising

a tray having a front wall and a back wall,

gate means mounted on each side of the tray for defining an enclosure within said walls for the agarose gel,

means positioned in said tray for defining wells in the agarose gel for DNA fragments,

an electrophoresis chamber having a platform and a buffer well on each side of the platform, said gate means being movable to positively locate the tray on said platform,

means in said chamber for establishing an electric field across said tray,

and means for illuminating said tray to visually observe the migration of said DNA fragments in the agarose gel.

2. The apparatus according to claim 1 wherein said illuminating means includes a normally open switch, a viewing cover mounted on said illuminating means for filtering UV radiation, and

means on said cover for closing said switch when the cover is properly located on the transilluminator.

3. The apparatus according to claim 2 wherein said transilluminator includes a glass frame mounted on said transilluminator, said frame having an inner peripheral surface to positively locate the tray on the transilluminator.

4. The apparatus according to claims 1, 2 or 3 including a camera assembly having a fixed focal length for providing an exact image of said DNA fragments.

5. The combination of an electrophoretic chamber, a transilluminator and a tray for holding agarose gel containing DNA fragments,

said chamber including a platform for supporting said tray and a well on each side of said platform for a buffer, said tray including means for forming an enclosure for said gel in one position and for positively locating the tray on the platform in the other position,

means in said chamber for establishing an electrical field across said tray to effect a migration of said DNA fragments into the agarose gel,

and said transilluminator including means for defining a viewing opening for illuminating said tray on said transilluminator.

6. The combination according to claim 5, including a camera assembly adopted to be mounted on said glass frame, said camera assembly including a hood having a fixed focal length for providing an exact picture of the DNA fragment migration patterns in said gel.

7. The combination according to claim 5 or 6, including a cover formed of a UV filter material mounted on said transilluminator in a position to enclose said viewing open defining means, whereby a tray placed in said viewing opening means can be observed without protective equipment.

8. The combination according to claim 7 wherein said transilluminator includes a normally open magnetically operated switch operatively connected to energize the transilluminator and said cover includes a magnetic member mounted on said cover for closing said switch when the cover is closed to energize said transilluminator.

9. The combination of an electrophoretic chamber, a transilluminator and a tray for holding agarose gel containing DNA fragments,

said chamber including a platform for supporting said tray and a well on each side of said platform for a buffer, said tray including a front wall, a back wall and a gate on each side, said gates being movables between a first position for forming an enclosure for said gel, and a second position for positively locating the tray on the platform in said chamber,

means in said chamber for establishing an electrical field across said tray to effect a migration of said DNA fragments into the agarose gel,

and said transilluminator including a UV light source and a frame having an inner peripheral surface for defining a viewing opening for positively locating said tray over said light source in said transilluminator, and an outer peripheral surface.

10. The combination according to claim 9, including a camera assembly including a UV filter hood having an opening for engaging the outer peripheral surface of said glass frame, said hood having a fixed focal length for providing an exact picture of the DNA fragment migration pattern in said gel.

11. The combination according to claim 9 or 10, including a cover formed of a UV filter material mounted on said transilluminator in a position to enclose said viewing opening in said frame.

12. The combination according to claim 11 wherein said transilluminator includes a normally open magnetically operated switch mounted in said transilluminator and being connected to de-energize said transilluminator when said cover is open and said cover includes a magnet for closing said switch when the cover is closed.

16. The transilluminator according to claims 12, 13 or 14 wherein said transilluminator includes a normally open magnetically operated switch mounted in said transilluminator and being connected to de-energize said transilluminator when the cover is open and said cover includes a magnet for closing said switch when the cover is closed.

13. A transilluminator for viewing a tray containing DNA fragment migration patterns in agarose gel, said transilluminator comprising

a housing for a UV light source,

and a cover formed of a UV filter material mounted on said housing in a position to cover said light source whereby a tray positioned over said light source can be viewed through said cover without protective glasses.

14. The transilluminator according to claim 13 including a frame for defining a viewing opening on said transilluminator and having an inner peripheral configuration corresponding to the outer configuration of said tray for locating said tray in said viewing opening.

15. The transilluminator according to claim 13 or 14 wherein said frame includes an outer peripheral surface corresponding to the inner peripheral surface of a camera hood for locating a camera over said viewing opening.

17. The combination of an electrophoretic chamber and a tray for holding an agarose gel containing DNA fragments, said chamber having a platform for supporting said tray, a buffer well on each side of said platform and means for establishing an electric field across said platform,

said tray including means for centering said tray on said platform.

18. The combination according to claim 17 wherein said centering means comprises a gate on each side of said tray mounted for movement between a first position for enclosing said tray and a second position for centering said tray on said platform.