Immunoassays utilizing covalent conjugates of polymerized enzyme and antibody
First Claim
Patent Images
1. A method for producing a polymeric-enzyme/antibody conjugate, comprising:
- (1) covalently coupling at least two enzyme molecules to produce a prepolymerized enzyme; and
(2) coupling covalently the prepolymerized enzyme to an antibody or fragment thereof.
14 Assignments
0 Petitions
Accused Products
Abstract
An enzyme is cross linked to form a polymerized enzyme which is covalently coupled to an antibody or antibody fragment. The use of the antibody-polymeric enzyme conjugate in immunoassays provides enhanced signal generation and, therefore, short assay time and high sensitivity.
52 Citations
22 Claims
-
1. A method for producing a polymeric-enzyme/antibody conjugate, comprising:
-
(1) covalently coupling at least two enzyme molecules to produce a prepolymerized enzyme; and (2) coupling covalently the prepolymerized enzyme to an antibody or fragment thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
-
-
16. In an immunoassay for the determination of an analyte in a liquid sample, said immunoassay comprising:
-
(1) contacting the sample with an enzyme-labeled anti-analyte antibody to form (i) a complex comprising enzyme-labeled anti-analyte antibody and analyte and (ii) free enzyme-labeled anti-analyte antibody; (2) separating the complex from the free enzyme-labeled anti-analyte antibody; (3) measuring the enzymatic activity of either the complex or the free enzyme-labeled anti-analyte antibody; and (4) relating the enzymatic activity to the amount of analyte initially present in the sample, the improvement comprising; using as the enzyme-labeled anti-analyte antibody, a covalent conjugate of a prepolymerized enzyme and an antibody or fragment thereof. - View Dependent Claims (17)
-
- 18. A conjugate comprising a prepolymerized enzyme comprising at least two enzyme molecules covalently coupled, said prepolymerized enzyme covalently coupled to an antibody or fragment thereof.
Specification