Support for solid-phase oligonucleotide synthesis
First Claim
1. A polymer support/nucleoside-linker composite having the general formula
wherein P'"'"' is a polymer support which bears oxirane, aziridine or episulfide groups;
S'"'"' is a nucleoside-linker having the general formula
space="preserve" listing-type="equation">W--(CH.sub.2).sub.a --X--(CH.sub.2).sub.b --Y--(CH.sub.2).sub.c --Zwherein W and Z each independently comprise a nucleophile selected from the group consisting of amine, alcohol and thio groups;
X and Y, which independently may or may not be present, comprise groups of high hydrophilicity; and
a, b, c are integers from 0 to 9, where a plus b plus c exceeds 6;
wherein P'"'"' is conjugated through a carbon thereof to S'"'"' by means of a covalent bond with either of said nucleophile W or Z.
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Accused Products
Abstract
This invention relates to a support for oligonucleotide synthesis and more particularly to a necleoside-linker/polymer support composite having the general formula
P'"'"'--S'"'"'
wherein P'"'"' is a polymer support which bears oxirane, aziridine or episulfide groups or which contains good leaving groups for nucleophilic displacement; and S'"'"' is a nucleoside-linker having the general formula
W--(CH.sub.2).sub.a --X--(CH.sub.2).sub.b --Y--(CH.sub.2).sub.c --Z
wherein W and Z each independently comprise a nucleophile; X and Y which,
independently may or may not be present, comprise groups of high hydrophilicity; and a, b, c are integers from 0 to 9, wherein a plus b plus c exceeds 6.
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Citations
22 Claims
- 1. A polymer support/nucleoside-linker composite having the general formula
- space="preserve" listing-type="equation">P'"'"'--S'"'"'
wherein P'"'"' is a polymer support which bears oxirane, aziridine or episulfide groups;
S'"'"' is a nucleoside-linker having the general formula
space="preserve" listing-type="equation">W--(CH.sub.2).sub.a --X--(CH.sub.2).sub.b --Y--(CH.sub.2).sub.c --Zwherein W and Z each independently comprise a nucleophile selected from the group consisting of amine, alcohol and thio groups;
X and Y, which independently may or may not be present, comprise groups of high hydrophilicity; and
a, b, c are integers from 0 to 9, where a plus b plus c exceeds 6;
wherein P'"'"' is conjugated through a carbon thereof to S'"'"' by means of a covalent bond with either of said nucleophile W or Z.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- 9. A support for solid phase oligonucleotide synthesis comprising a polymer support/nucleoside-linker/nucleoside composite having the general formula
- space="preserve" listing-type="equation">P'"'"'--S'"'"'--N'"'"'
wherein P'"'"' is a polymer support which bears oxirane, aziridine or episulfide groups;
S'"'"' is a nucleoside-linker having the general formula
space="preserve" listing-type="equation">W--(CH.sub.2).sub.a --X--(CH.sub.2).sub.b --Y--(CH.sub.2).sub.c --Zwherein W and Z each independently comprise a nucleophile selected from the group consisting of amine, alcohol and thio groups;
X and Y, which independently may or may not be present, comprise groups of high hydrophilicity; and
a, b, c are integers from 0 to 9, where a plus b plus c exceeds 6;
N'"'"' is a protected nucleoside or its analog; and
wherein P'"'"' is conjugated through a carbon thereof to S'"'"' by means of a covalent bond with either of said nucleophile W or Z.- View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
Specification