Synchronous wavelength drive and data acquisition conversion for a sequential spectrophotometer

• US 4,660,977 A
• Filed: 02/22/1985
• Issued: 04/28/1987
• Est. Priority Date: 02/22/1985
• Status: Expired due to Term

• Current Assignee: Perkin-Elmer Llc (Revvity, Inc.)
• Original Assignee: The Perkin-Elmer Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Wittmer, Charles M.
• Primary Examiner(s): Evans, F. L.
• Application Number:
  US06/704,344
• Time in Patent Office: 795 Days
• Field of Search: 356/319, 356/323, 356/325, 356/326, 356/328, 356/331, 356/334, 364/498
• US Class Current: 356/319
• CPC Class Codes: G01J 2003/066 Microprocessor control of functions, e.g. slit, scan, bandwidth during scan
• CPC Class Codes: G01J 3/06 Scanning arrangements arrangements for order-selection
• CPC Class Codes: G01J 3/42 Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry beam switching arrangements G01J3/08

Abstract

A sequential spectrophotometer system having a light source, a scanning type monochromator, a stepper motor means for changing the monochromator wavelength over a light spectrum being scanned, a photomultiplier for measuring the intensity of light absorption or passage through a sample being tested and generating a signal corresponding thereto, data acquisition means for compiling data corresponding to said signals and means for recording such data generally as a function of light measured for each wavelength, the improvement comprising:

a method and means to effect the data acquisition conversion at or about each desired wavelength during a respective stepper motor rotor displacement period, i.e., during each corresponding period of diffraction grating vibration or oscillation following a stepper motor disposition thereof to another wavelength position angle.

Claims

1. A sequential spectrophotometer system having a light source, a scanning monochromator device having a movable prism, a stepper motor coupled to the monochromator for periodically changing the monochromator device wavelength, a photomultiplier for detecting the intensity of the output light from the monochromator device and providing an electrical signal corresponding thereto, data acquisition means responsive to the electrical signal from the photomultiplier for measuring same, and a recording device for providing indicia representative of the measured electrical signal, the improvement comprising:

means for effecting a data acquisition during a prism displacement.

2. A sequential spectrophotometer system as in claim 1, wherein:

the means for effecting a data acquisition during a prism displacement is synchronized to the line frequency whereby said data acquisition occurs during a cycle of said line frequency.

3. A sequential spectrophotometer system as in claim 2, wherein:

the line frequency is approximately 60 hertz.

4. A sequential spectrophotometer system as in claim 1, wherein:

the means for effecting a data acquisition during a prism displacement measures light intensity of a band of wavelengths about a monochromatic wavelength, said band of wavelength being dependent on the amplitude of oscillations of the monochromator prism about a predetermined wavelength disposition of the monochromator prism.

5. A sequential spectrophotometer system as in claim 1, wherein:

the means for effecting a data acquisition during a prism displacement effects a wavelength change prior to termination of the prism displacement.

6. A sequential spectrophotometer system as in claim 1, wherein:

the means for effecting a data acquisition during a prism displacement being synchronized to the line frequency for causing the recording device to display indicia representative of an integrated electrical signal being measured during a portion of time when the line frequency goes from above to below its zero axis crossing.

7. A sequential spectrophotometer system as in claim 1, wherein:

the means for effecting a data acquisition during a prism displacement provides phase integrated data acquisition to enable equal signal integrate ratios on both sides of a zero axis crossing of an input power line frequency whereby spurious noise is substantially reduced for the acquisition data.

8. A sequential spectrophotometer system as in claim 1, wherein:

the means for effecting data acquisition includes a phase lock loop frequency multiplier, a microprocessor, and data acquisition electronic means for synchronizing both the data acquisition means and the stepper motor sequential wavelength change with the input power line frequency.

9. A sequential spectrophotometer system being powered from a line frequency power source comprising:

means responsive to the line frequency for providing a plurality of harmonic signal frequencies of the line frequency;

means responsive to said harmonic signal frequencies for providing a plurality of control signals;

means responsive to a respective one of said control signals for providing a drive signal;

stepper motor means being synchronized to the line frequency and responsive to said drive signal for incrementally rotating its rotor;

a source of light;

means responsive to said light and the rotational disposition of the rotor for providing a diffracted light generally corresponding with the rotational disposition of the rotor;

means responsive to said diffracted light for providing a corresponding electrical signal;

means synchronized to the line frequency and responsive to said control signals and said electrical signal for providing a data acquisition conversion signal representative of said diffracted light during a rotor displacement; and

means responsive to said data acquisition conversion signal for providing an indicia representative thereof;

whereby data acquisition conversion being effected during a rotor displacement.

10. A sequential spectrophotometer system as in claim 9, wherein:

the means responsive to the line frequency includes a phase lock loop frequency multiplier;

the harmonic responsive means includes a microprocessor;

the means providing the drive signal includes stepper motor drive electronics;

the means for providing the diffracted light includes a monochromator having a prism;

the means for providing the electrical signal includes a photomultiplier;

the means for providing the data acquisition conversion signal includes data acquisition electronics operatively coupled to said microprocessor and said photomultiplier for effecting data conversion;

whereby the data conversion is effected with said prism undergoing a prism displacement.

11. A synchronous wavelength drive and data acquisition conversion circuit network for a sequential spectrophotometer system being operated from a line frequency power source, said spectrophotometer having a plotter-scriber, a stepper motor, a monochromator having a prism, a source of light and a photomultiplier, comprising:

data acquisition means coupled to the photomultiplier for providing data conversion synchronized to the line frequency; and

control mans for synchronizing the data conversion with prism displacement of the monochromator prism to effect data conversion during a line frequency synchronized relatively small portion of the prism displacement occurring shortly after a wavelength change and within a respective cycle time of the line frequency.

12. A method of synchronizing the data conversion of a spectrophotometer system having a diffraction prism and a stepper motor operatively coupled to the diffraction prism with the line frequency, comprising the steps of:

providing a plurality of drive pulses to the stepper motor each being synchronized to a first corresponding portion of each respective cycle of the line frequency;

providing a plurality of data conversion command pulses to the data acquisition means of the spectrophotometer system each being synchronized to a second corresponding portion of each cycle of the line frequency; and

effecting a respective data conversion in response to each data conversion command pulse during a corresponding portion of a respective prism displacement.