Chemo-receptive lipid based membrane transducers

• US 4,661,235 A
• Filed: 08/03/1984
• Issued: 04/28/1987
• Est. Priority Date: 08/03/1984
• Status: Expired due to Fees

• Current Assignee: University of Toronto Innovations Foundation of Toronto The Metropolitan Toronto Ontario Canada
• Original Assignee: Michael Thompson, Ulrich J. Krull
• Inventors: Thompson, Michael, Krull, Ulrich J.
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/637,565
• Time in Patent Office: 998 Days
• Field of Search: 204/415, 204/418, 204/403, 204/1 N, 204/414, 204/1 T, 204/1 A, 435/817, 435/291, 435/4, 435/7, 435/11
• US Class Current: 204/403.06
• CPC Class Codes: G01N 27/3335 the membrane containing at least one organic component G01N27/3271 takes precedence; aspects concerning the enzyme reagent in enzyme electrodes C12Q1/001
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A lipid membrane is used as a chemo-receptive transducer for quantitative and semi-quantitative analysis of chemical test species in an electrolyte. The lipid membrane is contacted with an electrolyte containing the test species, and an electrical potential difference is applied across the membrane. From measurements of the trans-membrane ion current variation due to the presence of the test species, a quantitative analysis of the test species may be conducted, on a highly selective basis. Receptors showing a high degree of selectivity towards the test species are included in the membrane.

Claims

1. A lipid membrane-based device that utilizes transmembrane ion movement for analyzing the concentration of a selected chemical species,

said device comprising a perturbable, ion-permeable, lipid membrane and

an ion conductive support on which is deposited said ion-permeable lipid membrane, said ion-permeable lipid membrane including a complexing agent for selectively interacting with said selected chemical species to increase transmembrane ion movement, and said ion-permeable lipid membrane having an increased transmembrane ion movement when perturbed.

2. The supported, membrane-based device of claim 1, wherein said ion conductive support is a stable gel substrate.

3. The supported, membrane-based device of claim 1, wherein said ion conductive support is a hydrogel.

4. The supported, membrane-based device of claim 3, wherein said hydrogel is physically stabilized on an electrochemical reference electrode.

5. The supported, membrane-based device of claim 4, wherein said hydrogel is a polyacrylamide hydrogel.

6. The supported, membrane-based device of claim 1, wherein said lipid membrane is a bilayer lipid membrane.

7. The supported, membrane-based device of claim 1, wherein said lipid membrane is a monolayer lipid membrane.

8. The supported, membrane-based device of claim 1, wherein said complexing agent is a receptor selective for an organic compound.

9. The supported, membrane-based device of claim 8, wherein said receptor is a natural product.

10. The supported, membrane-based device of claim 8, wherein said receptor is chemically bound in the membrane.

11. The supported, membrane-based device of claim 1, wherein said complexing agent is selective for an inorganic ion.

12. A gas-sensor electrochemical cell comprising

(a) a gas-permeable membrane permeable to an inorganic ion-forming gas, and

(b) a perturbable, ion-permeable lipid membrane including a complexing agent for selectively interacting with a selected inorganic ion formed by dissolution of said inorganic ion-forming gas in an aqueous electrolytic solution,

said gas-permeable membrane being separated from said ion-permeable lipid membrane by the lipid membrane-contacting, aqueous electrolytic solution.

13. The gas-sensor electrochemical cell of claim 12, wherein said complexing agent is a polypeptide.

14. The gas-sensor electrochemical cell of claim 13, wherein said polypeptide is an antibiotic selected from the group consisting of gramicidin, valinomycin and nonactin.

15. The gas-sensor electrochemical cell of claim 13, wherein said polypeptide is nonactin.

16. The gas-sensor electrochemical cell of claim 12, wherein said gas-permeable membrane is a polytetrafluoroethylene membrane.

17. The gas-sensor electrochemical cell of claim 12, wherein said modified membrane is a bilayer lipid membrane.

18. The gas-sensor electrochemical cell of claim 12, wherein said modified membrane is a monolayer lipid membrane.