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Competitive ELISA for the detection of HTLV-III antibodies

  • US 4,661,445 A
  • Filed: 05/24/1985
  • Issued: 04/28/1987
  • Est. Priority Date: 05/24/1985
  • Status: Expired due to Fees
First Claim
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1. A method for the detection of HTLV-III antibodies in a test sample comprisingcoating microtiter plates with purified HTLV-III antigen to form antigen-coated wells;

  • incubating a portion of said antigen coated wells with heterologous anti-HTLV-III;

    adding test sera to said wells;

    screening said wells for the amount of binding of HTLV-III antibodies by comparing said wells containing said heterologous anti-HTLV-III to control wells, said control wells being incubated without said heterologous anti-THLV-III.

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