Methods for immobilizing and translocating biological cells

• US 4,661,451 A
• Filed: 02/06/1984
• Issued: 04/28/1987
• Est. Priority Date: 02/06/1984
• Status: Expired due to Term

• Current Assignee: Ortho Diagnostic Systems, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Original Assignee: Ortho Diagnostic Systems, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Inventors: Hansen, W. Peter
• Primary Examiner(s): Marantz, Sidney
• Assistant Examiner(s): Krawczewicz, Louanne C.
• Application Number:
  US06/577,120
• Time in Patent Office: 1,177 Days
• Field of Search: 435/173, 435/174, 435/176-182, 435/288, 424/3, 204/180 R, 204/299 R, 204/403, 210/748, 210/243, 436/63, 436/177, 436/178, 422/101, 209/127 R, 219/203, 219/212
• US Class Current: 435/174
• CPC Class Codes: C12N 11/00 Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

Apparatus for immobilizing biological cells based on the dielectric properties of biological cells. An inhomogeneous electric field emanating from a grid point location or other contact area is created and attracts the biological cell into contact therewith. Appropriate controls over a series of grid points permits controlled inter-grid point movement of cells thereby permitting sequential testing or sorting processes to be carried out.

Claims

1. An apparatus for immobilizing biological cells comprising:

(a) an insulating substrate having a plurality of conducting contact areas mounted thereon;

(b) an alternating current source having two outputs, one of said outputs connected to ground; and

(c) connecting means for coupling the other of said current source outputs to said contact areas whereby a nonuniform electric field is produced emanating from said plurality of contact areas and capable of attracting and holding biological cells suspended in a conducting solution in contact with said plurality of contact areas, said connecting means further comprising means for controlling the connection between said contact areas and said current source whereby said cells may be released from said contact areas and, wherein said means for controlling includes means for disconnecting at least one contact area, independent of other contact areas, from said alternating current source whereby at least one predetermined cell at said one contact area may be selectively released from said one contact area while cells at other contact areas remain immobilized.

2. The apparatus as described in claim 1 further comprising electronic means for stimulating whereby at least one contact area may be separately stimulated for altering cellular characteristics of a cell associated with said stimulated contact area.

3. The apparatus as described in claim 1 wherein said contact areas are elongated and arranged in a pattern.

4. A method for immobilizing biological cells for subsequent testing or culturing comprising the steps of:

(a) providing the biological cells to be immobilized in a solution capable of supporting an inhomogeneous electric field and maintaining cellular viability for a time at least sufficient to conduct the desired testing or culturing of said cells; and

(b) bringing said biological cells in contact with an apparatus comprising;

(i) an insulating substrate having a plurality of conducting contact areas mounted thereon;

(ii) an alternating current source having two outputs, one of said outputs connected to ground; and

(iii) connecting means for coupling the other of said current source outputs to said contact areas whereby a nonuniform electric field is produced emanating from said plurality of contact areas and capable of attracting and holding biological cells suspended in a conducting solution in contact with said plurality of contact areas, wherein said connecting means coupling said current source to said contact area further comprises control means for independently connecting said current source to said contact areas;

(c) energizing said current source connected to said contact areas for creating an inhomogeneous electric field whereby said biological cells are immobilized on said contact areas.

5. The method of claim 4 wherein said energizing step further includes the step of directing cellular movement between contact areas.