Microorganism monitoring apparatus

• US 4,661,845 A
• Filed: 12/24/1984
• Issued: 04/28/1987
• Est. Priority Date: 12/23/1983
• Status: Expired due to Fees

• Current Assignee: Nishihara Environmental Sanitation Research Corporation Limited
• Original Assignee: Nishihara Environmental Sanitation Research Corporation Limited
• Inventors: Saito, Toshio, Yukawa, Kazuo, Suzuki, Tomio
• Primary Examiner(s): Britton, Howard W.
• Assistant Examiner(s): Kostak, Victor R.
• Application Number:
  US06/685,309
• Time in Patent Office: 855 Days
• Field of Search: 358/99, 358/100, 358/93, 358/107, 358/901, 422/94, 422/95, 422/96.1, 435/291, 435/808, 350/536, 350/582, 350/584, 350/319, 356/244, 356/246, 250/576
• US Class Current: 348/81
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404
• CPC Class Codes: G01N 21/8507 Probe photometers, i.e. with optical measuring part dipped into fluid sample

Abstract

An apparatus for directly monitoring microorganisms present in a liquid crises a window glass with one face in contact with the liquid. Near the other face is disposed a TV camera having an optical magnifying system for monitoring the microorganisms. A sample cell or a narrow space is formed on said one side of the window glass for direct introduction of a sample of the liquid. The sample is statically held in the cell even though the liquid is in movement. The sample is purged from the cell by feeding a cleaning fluid thereinto or by vibrating or otherside exerting physical movement on the cell, whereafter a new sample is introduced into the cell. Purging of the sample cleans the cell and is prevents it from being smudged with dregs, dirt or the like included in the liquid.

Claims

1. An apparatus for directly monitoring microorganisms present in a liquid, comprising:

transparent window means, one face of which is adapted to be in contact with the liquid;

a plate member disposed opposite to the one face of said transparent window means so as to form a narrow sample cell between said one face and a surface of said plate member, said sample cell being adapted to be submerged in the liquid to be monitored and having an opening to permit introduction and discharge of a sample from the liquid thereinto;

a tube member, one end of which is connected to said sample cell and the other end of which can be connected to a source for a cleaning fluid to purge the sample held therein;

a light source for directing a light beam onto the sample held in said sample cell;

an optical magnifying system disposed opposite to the other face of said transparent window means to magnify an image of said microorganisms in the sample illuminated by said light beam; and

means for observing the image magnified by said optical magnifying system.

2. An apparatus as set forth in claim 1, wherein said plate member has reflector means provided on its face opposed to the one face of said transparent window means, said light source is provided on the side of the other face of said transparent window means, the light beam emitted from said light source is guided through said optical magnifying system toward said reflector means, and said optical magnifying system receives the light beam reflected by said reflector means.

3. An apparatus as set forth in claim 1, wherein said light source is provided on the face of said plate member which is opposed to the one face of said transparent window means, and said optical magnifying system receives the light beam from said light source through said transparent window means.

4. An apparatus as set forth in claim 2, wherein said observing means includes a TV camera and a display device connected thereto, said transparent window means is mounted on a wall of a closed housing which is adapted to be submerged in the liquid, and said TV camera, said light source, said optical magnifying system and said tube member are housed within said closed housing.

5. An apparatus as set forth in claim 3, wherein said observing means includes a TV camera and a display device connected thereto, said transparent window means is mounted on a housing wall of a closed housing which is adapted to be submerged in the liquid, and said TV camera, said optical magnifying system and said tube member are housed within said closed housing.

6. An apparatus as set forth in claim 4, wherein said optical magnifying system is combined with said TV camera as a part thereof, the magnifying power of said optical magnifying system is adjustable, and said reflector means is movable to adjust the space between said reflector means and the one face of said transparent window means in response to the adjustment of the magnifying power.

7. An apparatus as set forth in claim 6, wherein said TV camera with the optical magnifying system is movable within a plane parallel with said transparent window means.

8. An apparatus as set forth in claim 7, wherein said TV camera is further movable within a plane perpendicular to said transparent window means.

9. An apparatus as set forth in claim 1, wherein the liquid is contained in a vessel, said transparent window means is mounted on the wall of said vessel.

10. An apparatus as set forth in claim 9, wherein said transparent window means comprises a cylindrical optical glass which extends through the wall of said vessel and which guides an image from its one end face to its other end face.

11. An apparatus as set forth in claim 1, wherein said transparent window means comprises a flexible bundle of optical fibers the free end face of which is immersed into the liquid through the surface thereof.

12. An apparatus as set forth in claim 1, wherein the liquid is in a vessel for a bioreactor, said transparent window means is mounted on the wall of said bioreactor vessel, and a raw material to be fed into said bioreactor vessel, a product obtained from said bioreactor vessel and/or a liquid contained in said bioreactor vessel is used as the cleaning fluid for purging the sample held in said sample cell.

13. An apparatus set forth in claim 1, wherein said plate member is movable to adjust a space between said plate member and the one face of said transparent window.

14. An apparatus set forth in claim 2, wherein said reflector means is movable to adjust a space between said plate member and the one face of said transparent window.

15. An apparatus for directly monitoring microorganisms present in a liquid, comprising:

transparent window means, one face of which is adapted to be in contact with the liquid;

a plate member with a magnet disposed opposite to the one face of said transparent window means and being movable perpendicularly to said one face;

spacer means provided between said plate member and the one face of said transparent window means so as to form a narrow intervening space for introducing a sample from the liquid thereinto;

an electromagnetic coil provided on the side of the other face of said transparent window means to move said plate member perpendicularly to the one face of said transparent window means for purging the sample held in said narrow space and for introducing a new sample thereinto;

a light source for directing a light beam onto the sample held in said narrow space;

an optical magnifying system disposed opposite to the other face of said transparent window means to magnifying an image of the sample illuminated by said light beam; and

means for observing the image magnified by said optical magnifying system.

16. An apparatus as set forth in claim 15, wherein said spacer means comprises at least three projections formed on the circumference of said transparent window means.

17. An apparatus as set forth in claim 15, wherein said spacer means comprises at least three projections formed on said plate member.

18. An apparatus as set forth in claim 15 wherein said plate member has reflector means provided on its face opposed to the one face of said transparent window means, said light source is disposed on the side of said transparent window means, the light beam emitted from said light source is guided through said optical magnifying system toward said reflector means, and said optical magnifying system receives the light beam reflected by said reflector means.

19. An apparatus as set forth in claim 15 wherein said light source is provided on the face of said plate member which is opposed to the one face of said transparent window means, and said optical magnifying system receives the light beam from said light source through said transparent window means.

20. An apparatus as set forth in claim 18, wherein said observing means includes a TV camera and a display device connected thereto, said transparent window means is mounted on a wall of a closed housing which is adapted to be submerged in the liquid, and said TV camera, said light source and said optical magnifying system are housed within said closed housing.

21. An apparatus as set forth in claim 19, wherein said observing means includes a TV camera and a display device connected thereto, said transparent window means is mounted on a wall of a closed housing which is adapted to be submerged in the liquid, and said TV camera and said optical magnifying system are housed within said closed housing.

22. An apparatus as set forth in claim 15, wherein the liquid is contained in a vessel, said transparent window means is mounted on the wall of said vessel.

23. An apparatus as set forth in claim 18, wherein said transparent window means is mounted on the wall of said vessel.

24. An apparatus as set forth in claim 19, wherein said transparent window means is mounted on the wall of said vessel.

25. An apparatus as set forth in claim 15, wherein said transparent window means comprises a flexible optical image guide the free end face of which is immersed into the liquid through the surface thereof.

26. An apparatus as set forth in claim 18, wherein said transparent window means comprises a flexible bundle of optical fibers the free end face of which is immersed into the liquid through the surface thereof.

27. An apparatus as set forth in claim 19, wherein said transparent window means comprises a flexible bundle of optical fibers, a free end face of said bundle being immersed into the liquid through the surface thereof.

31. An apparatus as set forth in claim 26, wherein said purging and introducing means comprises a tube member one end of which is connected to said sample cell and the other end of which can be connected to a source for a cleaning fluid to purge the sample held therein.

32. An apparatus as set forth in claim 31, wherein said tube member is housed in said elongate housing.

34. An apparatus as set forth in claim 31, wherein said elongate housing, said light guide, said optical image guide and said tube member are flexible.

28. An apparatus for directly monitoring microorganisms present in a liquid, comprising:

an elongate housing including a light guide and an optical image guide therewithin and having a free end face adapted to be in contact with the liquid, said light guide and said optical image guide extending to the free end face of said elongate housing so that respective free end faces of said light guide and said optical image guide are exposed at the free end face of said elongate housing;

a plate member formed as an extended portion of the free end of said elongate housing and disposed opposite to the free end face of said elongate housing so as to form a sample cell there between, a part of said sample cell being open to permit introduction of a sample from the liquid thereinto;

a refractor means supported by said plate member and having a flat face which is opposed to the free end face of said optical image guide so as to form a narrow space therebetween;

a light source provided on the other end face of said light guide;

said refractor means being arranged in such a manner that after a light beam emitted from said light source passes through said light guide, it is introduced into said optical image guide through the exposed end face thereof;

an optical magnifying system provided on the other end face of said optical image guide to magnifying an image of the sample illuminated has the light beam introduced into said optical image guide;

means for observing the image magnified by said optical magnifying system; and

means for purging the sample held in said sample cell and for introducing a new sample thereinto.

29. An apparatus as set forth in claim 28, wherein said purging and introducing means comprises a ultrasonic vibrator provided in said sample cell.

30. An apparatus as set forth in claim 28, wherein said purging and introducing means comprises a motor for rotating said refractor means.

33. An apparatus as set forth in claim 28, wherein said elongate housing, said light guide and said optical image guide are flexible.