Gas flow cartridge having resilient flexible membrane with slit separating reaction and reagent chambers

• US 4,663,127 A
• Filed: 10/15/1982
• Issued: 05/05/1987
• Est. Priority Date: 10/15/1982
• Status: Expired due to Term

• Current Assignee: Hemotec Incorporated (Medtronic Plc)
• Original Assignee: Hemotec Incorporated (Medtronic Plc)
• Inventors: Dvornichenko, Anatoly, Cooper, Daniel, Jackson, Jacqueline J.
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/434,569
• Time in Patent Office: 1,663 Days
• Field of Search: 422/100, 422/101, 422/102, 422/103, 422/73, 422/57, 422/58, 422/61, 422/119, 422/44, 422/47, 436/69, 436/165, 436/809, 356/39, 73/64.1, 73/57, 435/13, 210/918
• US Class Current: 422/417
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/56
• CPC Class Codes: G01N 33/86 involving blood coagulating time or factors, or their receptors

Abstract

A gas flow cartridge in which to conduct analytical coagulation tests on a sample of fluid such as blood includes a tube-like member and a resilient flexible membrane having a slit formed therethrough to divide the tube-like member into an upper reaction chamber in which the test takes place and into a lower reagent chamber which initially contains reagent to be mixed with the sample of liquid upon which the test is to be conducted. The contents of the reagent chamber are forced through the slit in the resilient flexible membrane due to the flexing of the membrane around the slit when pressure is applied. Once the reagent is in the reaction chamber with the sample of fluid, a flow of gas is forced upward through the slit in the membrane during the analytical test.

Claims

1. A cartridge in which to conduct an analytical test on fluid material inserted therein, the analytical test including the step of flowing pressurized gas into the cartridge to operatively interact the gas with the fluid material during the course of the analytical test, said cartridge comprising:

a tube-like member having an open interior extending generally axially therethrough from a lower end to an upper end;

dividing means within the open interior of the tube-like member at a position intermediate the upper and lower ends for dividing the tube-like member into a reagent chamber between said dividing means and the lower end and into a reaction chamber between said dividing means and the upper end, the reaction chamber adapted to receive and hold an inserted fluid material for interaction with gas during an analytical test;

a passageway defined by said dividing means and extending between the reagent and reaction chambers;

plug means operatively sealing the open interior of the reagent chamber at a position initially spaced from the dividing means and adjoining the lower end of the tube-like member, said plug means adapted for operative movement toward the dividing means to reduce the volume of the reagent chamber, said plug means further including means for admitting and conducting pressurized gas into the cartridge during the analytical test;

a quantity of fluid reagent initially confined within the reagent chamber; and

means for opening and closing the passageway in response to fluid pressure within the reagent chamber, said opening and closing means operatively (a) normally maintaining the passageway sealed to prevent the reagent from flowing into the reaction chamber prior to commencement of the analytical test, (b) opening the passageway in response to an increase in fluid pressure within the reagent chamber upon the upward movement of the plug means toward the dividing means, (c) maintaining the passageway open to conduct the fluid reagent therethrough into the reaction chamber as the plug means moves upward toward the dividing means, (d) opening the passageway in response to the presence of pressurized gas admitted at the plug means (e) maintaining the passageway open to conduct the admitted pressurized gas into the reagent chamber during the analytical test, and (f) sealing the passageway in response to the termination of the supply of pressurized gas at the conclusion of the analytical test to prevent the material in the reaction chamber from leaking through the passageway at the conclusion of the analytical test.

2. A cartridge as defined in claim 1 wherein said fluid pressure responsive means for opening and closing the passageway comprises a resilient flexible member having resilient characteristics which normally opens the passageway when relative pressure differential between the regent chamber and the reaction chamber exceeds a predetermined amount, and which flexes under the predetermined amount of pressure differential to open the passageway to the flow of fluid therethrough.

3. The cartridge as defined in claim 2 wherein the flexible member includes a normally closed opening which opens under the predetermined amount of pressure differential.

4. The cartridge as defined in claim 3 wherein the normally closed opening comprises a slit formed through the resilient flexible member.

5. A cartridge as defined in claim 3 wherein the resilient flexible member includes a slit formed therethrough, and the resiliency of the flexible member normally holds the slit in a closed position to seal the slit against the passage of fluid therethrough, and a portion of the member adjacent the slit resiliently flexes under the influence of the predetermined fluid pressure to open the slit and allow fluid communication therethrough.

6. A cartridge as defined in claim 5 wherein said plug means further comprises a dome portion formed of resilient flexible material, and said means for admitting and conducting pressurized gas into the cartridge includes a slit formed through the dome material.