Process for enhanced fermentation of xylose to ethanol

• US 4,663,284 A
• Filed: 09/14/1984
• Issued: 05/05/1987
• Est. Priority Date: 09/14/1984
• Status: Expired due to Fees

• Current Assignee: United States Department Of Agriculture
• Original Assignee: United States Department Of Agriculture
• Inventors: Jeffries, Thomas W.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): WHITE, PATRICIA K
• Application Number:
  US06/650,462
• Time in Patent Office: 963 Days
• Field of Search: 435/161, 435/162, 435/163, 435/165, 435/813, 435/911, 435/921, 435/255
• US Class Current: 435/161
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y10S 435/813 Continuous fermentation
• CPC Class Codes: Y10S 435/911 using fungi
• CPC Class Codes: Y10S 435/921 Candida

Abstract

Ethanol is produced from D-xylose by fermentation with any of the known xylose-metabolizing yeasts, such as Pachysolen tannophilus. To improve the yield of ethanol, small quantities of glucose are added to the fermentation medium during the fermentation process.

Claims

1. A process for the production of ethanol, which process comprises:

(a) aerobically fermenting D-xylose in a fermentation medium comprising D-xylose with a yeast having the ability to convert D-xylose to ethanol; and

(b) adding glucose to said fermentation medium after said fermenting has commenced and during said fermenting with the adding being continuously or by successive addition at intervals during said fermenting and with said intervals between successive addition being not greater than about 24 hours and wherein the amount of the glucose added continuously or by additions does not bring the glucose concentration to exceed about 1 percent by weight of the fermentation medium.

2. A process according to claim 1 wherein said adding is by said successive additions and said intervals between the successive additions of glucose are not greater than about twelve hours and the amount of glucose added to said fermentation medium at any one addition does not exceed about 0.5 percent by weight of said fermentation medium.

3. A process according to claim 1 wherein said yeast is selected from the group consisting of Candida spp., Kluyveromyces marxianus and Pachysolen tannophilus.

4. A process according to claim 1 wherein said yeast is Pachysolen tannophilus.

5. A process according to claim 1 wherein said D-xylose is present in the form of an acid or enzymatic hydrolysate of hemicellulose.

6. A process for the production of ethanol, which process comprises:

(a) fermenting a fermentation medium comprising D-xylose with Pachysolen tannophilus, having deposit accession numbers NRRL Y-2460 and ATCC 32691, and

(b) making a plurality of additions of glucose to said fermentation medium at intervals during said fermenting, the intervals between successive additions of glucose being not greater than about twelve hours and with the amount of glucose in each addition not exceeding about 0.5 percent by weight of said fermentation medium and being the amount providing a glucose concentration in said fermentation medium not exceeding about 0.5 percent of said fermentation medium.

7. A process for producing an enhanced ethanol yield by aerobically fermenting D-xylose in a fermentation medium with Pachysolen tannophilus having deposit accession numbers NRRL Y-2460 and ATCC 32691 wherein the improvement comprises adding glucose to said fermentation medium after said fermentation has commenced and during the carrying forth of said fermentation with the adding being continuously or at intervals during each 24 hours of the carrying forth of said fermentation and being of an amount of the glucose providing a glucose concentration not exceeding one percent by weight of the fermentation medium until substantially all D-xylose is consumed.