Multicuvette rotor for analyzer

• US 4,663,296 A
• Filed: 10/26/1983
• Issued: 05/05/1987
• Est. Priority Date: 05/05/1980
• Status: Expired due to Term

• Current Assignee: Hoffmann-La Roche Incorporated (Roche Holding AG)
• Original Assignee: Hoffmann-La Roche Incorporated (Roche Holding AG)
• Inventors: Thevoz, Michel, Revillet, Georges
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US06/545,709
• Time in Patent Office: 1,287 Days
• Field of Search: 422/72, 422/102, 422/64, 436/45, 436/177, 356/244, 356/246
• US Class Current: 436/45
• CPC Class Codes: B04B 5/0407 for liquids contained in receptacles B04B5/0442 takes precedence
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

The invention relates to an analytical rotor comprising a set of analytical cells disposed at its periphery, a central distribution chamber, portioning cavities corresponding to the respective cells and disposed between the cells and the chamber periphery, the cavities each having an inlet connected to the chamber and an outlet connected to one of the cells by a transfer passage, and at least one overflow reservoir connected to the inlets of the cavities by at least one communication passage.

Claims

1. A method for transferring by centrifugal force a pre-determined quantity of a liquid to an analytical cell which forms part of an analytical rotor wherein said rotor has a plurality of analytical cells located around the periphery of the rotor, each cell having only one aperture which is normally open and is the inlet of the cell, a central distribution chamber, and a plurality of concentrically located portioning cavities, each portioning cavity being adjacent to the central distribution chamber and an analytical cell, wherein the portioning cavity is located between the outer periphery of the central distribution chamber and the analytical cell, each portioning cavity being sized to receive the pre-determined quantity of liquid and having an inlet which connects it to the central distribution chamber and an outlet which connects it to the aperture, in the analytical cell which it is adjacent to, via a capilary passageway which opens into a vertical sidewall of the analytical cell, and the rotor further having at least one overflow reservoir for receiving excess liquid connected to the central distribution chamber by at least one connecting path, said method comprising the steps of:

(a) introducing a given quantity of liquid greater than the total volume of all the portioning cavities into the distribution chamber of the rotor;

(b) as a result of driving said rotor on a centrifuge at a first rotational speed to exert centrifugal force on the liquid introduced into the central distribution chamber, transferring the pre-determined quantity of liquid into each portioning cavity and into each capillary passageway, forming a meniscus at the end of each capillary passageway where it opens into the vertical wall of the analytical cell which it is connected to hermetically sealing the analytical cell with the meniscus so that air within the analytical cell can no longer flow out thereby creating a resistance to flow of the liquid into the analytical cell, and transfering excess liquid into the overflow reservoir; and

(c) then as a result of driving the rotor at a second rotational speed greater than the first rotational speed, breaking the meniscus which was formed in step (b) at the end of each of the capillary passageways and passing said pre-determined quantity of liquid from each portioning cavity through each corresponding capillary passageway into the analytical cell to which it is connected while allowing air which was trapped in the analytical cells to escape through each of the capillary passageways.

2. The method of claim 1 wherein the capillary passageway of the rotor has a cross-section chosen so that resulting capillary forces maintain a cohesive meniscus of the liquid at the outlet of the capillary passageway to the analytical cell when the rotor is driven at the first rotational speed and when the rotor is driven at the second rotational speed, the resulting contrifugal force breaks the meniscus and thereby permits the liquid to enter the analytical cell.

3. A method in accordance with claim 1, wherein the first rotational speed is about 400-600 rpm and the second rotational speed is about 4,000-5,000 rpm.