Fluorescent energy transfer with phycobiliproteins

• US 4,666,862 A
• Filed: 08/14/1984
• Issued: 05/19/1987
• Est. Priority Date: 08/14/1984
• Status: Expired due to Term

• Current Assignee: Ortho Diagnostic Systems, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Original Assignee: Ortho Diagnostic Systems, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Inventors: Chan, Joseph L. W.
• Primary Examiner(s): Kepplinger, Esther M.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US06/640,499
• Time in Patent Office: 1,008 Days
• Field of Search: 436/536, 436/501, 436/537, 436/546, 436/800, 436/816, 252/301.16, 424/11, 530/370
• US Class Current: 436/501
• CPC Class Codes: G01N 33/533 with fluorescent label
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine

Abstract

Dyes are provided comprising phycobiliproteins having covalently coupled thereto a plurality of small molecular weight organic dyes under conditions permitting energy transfer mechanisms to operate therebetween. The resultant label may then be used in virtually any immunoassay format.

Claims

1. In a method for the detection of a ligand by detecting an immunological reaction between said ligand and a ligand binding receptor specific therefor, the improvement comprising detecting said immunological reaction by detecting a label associated with one of said ligand or ligand binding receptor components, said label comprising a plurality of Azure A molecules covalently coupled to Allophycocyanin under conditions allowing energy transfer therebetween;

said detecting further comprising illuminating said label at a first frequency capable of exciting the Azure A molecules and detecting fluorescent emission from said Allophycocyanin at a second frequency.

2. A fluorescent label comprising a plurality of Azure A molecules covalently coupled to Allophycocyanin whereby excitation of said Azure A molecules results in energy transfer to said Allophycocyanin resulting in detectable fluorescent emission by said Allophycocyanin.