Cancer tests using tumor antigen generated lymphokines and compositions

• US 4,670,386 A
• Filed: 11/25/1983
• Issued: 06/02/1987
• Est. Priority Date: 05/23/1980
• Status: Expired due to Fees

• Current Assignee: Stephen Sugaar
• Original Assignee: Stephen Sugaar
• Inventors: Sugaar, Stephen
• Primary Examiner(s): Nucker, Christine M.
• Application Number:
  US06/554,925
• Time in Patent Office: 1,285 Days
• Field of Search: 435/617, 435/29, 435/68, 435/173, 435/182, 436/535, 436/543-546, 436/813, 436/829
• US Class Current: 435/29
• CPC Class Codes: G01N 33/574 for cancer

Abstract

The present invention provides strongly antigenic tumor-specific substances (also cellular material containing said strongly antigenic tumor-specific substances) prepared by radiofrequency or microwave electromagnetic radiation, acting on cancer tissue or cancer cells of known pathohistologic character. Said substances and also cellular material containing said substances are characterized by such sensitizing antigenic qualities that when they are contacted with a composition of naturally cancer-sensitized lymphocytes taken from a host having pathohistologically related cancer, then said lymphocytes produce and exude lymphokine.

The invention further provides diagnostic tests for cancer and compositions which contain or utilize said strongly-antigenic cancer-specific substances.

Claims

1. A process of forming strongly-antigenic tumor-specific substances in viable human cancer-tissue, cancer cells or cancer cell membranes in vitro by positioning such in radiofrequency or microwave radiation fields that causes changes of alignment of electric charges in all polar cellular components of said cancer tissue or cancer cells or cancer cell membranes at the very rapid rate of electric field changes in said radiation fields following which in said cancer tissue or cancer cells or cancer cell membranes strongly antigenic tumor-specific substances are formed.

2. Water-soluble extracts or water-soluble eluates removed or issuing forth, respectively, from cancer tissue or cancer cells produced by the process of claim 1.

3. A process according to claim 1 wherein said radiofrequency or microwave electromagnetic radiation is carried out by inductive or dielectric method.

4. A process for the formation and secretion of lymphokine by viable, in vitro cultured lymphocytes, taken from an individual having cancer comprising and exposing said lymphocytes to and culturing together in vitro in an aqueous cell-culture medium with eluate or extract from cancer tissue of claim 2 or irradiated cancer cells submitted to the process forming strongly-antigenic tumor-specific substances according to claim 1, when said cancer tissue or cancer cells are identical or related in pathohistological character to the cancer of said individual.

5. The process of claim 4 wherein said culturing is carried out for 24 to 72 hours at 37°

C. in germ-free condition in an atmosphere of 5% carbon dioxide in air, said culturing leading to secretion of lymphokine by lymphocytes.

6. Thin lipid layers of liposomes enclosing a water phase containing extracts or eluates of claim 2.

7. A laboratory diagnostic method for pathohistologically well-characterized forms of cancer comprising obtaining value lymphocytes from an individual tested for cancer of a definite pathohistological character,

contacting said viable lymphocytes in a watery culture medium with cancer cells produced by the process of claim 1 or eluate or extracts of cancer cells of claim 2, said cancer cells being of identical or related pathohistological character to the cancer for which said individual is being tested to form watery composites, and

culturing for 24 to 72 hours in vitro said watery composites in germ-free condition in a humid atmosphere containing 5% carbon dioxide in air,

whereupon secretion of lymphokine by said lymphocytes is a positive diagnostic sign for cancer of said pathohistological character affecting said individual.

8. A laboratory diagnostic method for well-characterized forms of cancer comprising obtaining viable lymphocytes from an individual being tested for cancer of a definite pathohistological character,

contacting said viable lymphocytes in a watery culture medium with liposomes comprising thin lipid layers enclosing a watery phase containing eluate or cancer cell extract produced according to claim 2 said cancer cells being of identical or related in pathohistological character to the cancer for which said individual is tested forming watery composites and

culturing in vitro said watery composites for 24 to 72 hours in germ-free condition in a humid atmosphere containing 5% carbon dioxide in air,

whereupon if said culturing leads to secretion of lymphokine from said lymphocytes, said individual has cancer of identical or related character as the pathohistologically known tumor-specific antigenic substance present in said liposomes watery phase.