Multilayer analytical element

• US 4,671,937 A
• Filed: 07/02/1984
• Issued: 06/09/1987
• Est. Priority Date: 06/30/1983
• Status: Expired due to Term

• Current Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Nagatomo, Shigeru, Arai, Fuminori, Katsuyama, Harumi, Miyazako, Takushi
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): DeSantis, Patricia L.
• Application Number:
  US06/626,743
• Time in Patent Office: 1,072 Days
• Field of Search: 436/518, 436/810, 436/904, 436/903, 436/910, 435/4, 435/22, 435/805, 422/56, 427/3
• US Class Current: 422/423
• CPC Class Codes: C12Q 1/40 involving amylase
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick
• CPC Class Codes: Y10S 436/903 Diazo reactions
• CPC Class Codes: Y10S 436/904 Oxidation - reduction indicators
• CPC Class Codes: Y10S 436/91 Iron-binding capacity of blood

Abstract

A multilayer analytical element for analysis of analyte in a liquid sample comprising a liquid sample-spreading layer, a reagent layer containing a diazonium salt, and a liquid-impermeable light-transmissive support, being laminated in this order, in which said reagent layer contains a sulfonic acid group-containing polymer having at least 10 molar % of a repeating unit selected from those consisting of a sulfoalkyl group and sulfophenyl group. The analytical element can be an element comprising a porous reagent layer containing a diazonium salt and the liquid-impermeable light-transmissive support, being laminated in this order.

Claims

1. A multilayer analytical element for analysis of a substance in a liquid sample comprising a liquid sample-spreading layer, a reagent layer containing a diazonium salt, and a liquid-impermeable light-transmissive support, in which said reagent layer contains a polymer containing at least 10 molar % of a repeating unit having a sulfophenyl group, wherein said polymer provides a pH environment which preserves the diazonium salt in the reagent layer.

2. The analytical element of claim 1 in which said polymer contains not less than 30 molar % of the repeating unit having a sulfophenyl group.

3. The analytical element of claim 1 in which said polymer is a homopolymer consisting essentially of a repeating unit having a sulfophenyl group.

4. A multilayer analytical element for the analysis of a substance in a liquid sample comprising a liquid sample-spreading layer, a reagent layer containing a diazonium salt and a liquid-impermeable light-transmissive support, in which said reagent layer contains a polymer containing at least 10 molar % of a repeating unit selected from those consisting of a repeating unit having a sulfoalkyl group and a repeating unit having a sulfophenyl group, and in which a nondiffusive base-containing layer is provided under the condition that the nondiffusive base-containing layer is in liquid contact with said reagent layer, and wherein said polymer provides a pH environment which preserves the diazonium salt in the reagent layer.

5. The analytical element of claim 4 in which said nondiffusive base is in the form of a polymer.

6. The analytical element of claim 4 in which said polymer contains not less than 30 molar % of the repeating unit selected from those consisting of a repeating unit having a sulfoalkyl group and a repeating unit having a sulfophenyl group.

7. The analytical element of claim 4 in which said polymer is a homopolymer consisting essentially of a repeating unit selected from those consisting of a repeating unit having a sulfoalkyl group and a repeating unit having a sulfophenyl group.

8. A multilayer analytical element for analysis of a substance in a liquid sample comprising a porous reagent layer containing a diazonium salt and a liquid impermeable light-transmissive support, in which said porous reagent layer contains a polymer containing at least 30 molar % of a repeating unit having a sulfophenyl group, and wherein said polymer provides a pH environment which preserves the diazonium salt in the reagent layer.

9. The analytical element of claim 8 in which said polymer is a homopolymer consisting essentially of a repeating unit having a sulfophenyl group.

10. A multilayer analytical element for analysis of a substance in a liquid sample comprising a porous reagent layer containing a diazonium salt and a liquid impermeable light-transmissive support, in which which said porous reagent layer contains a polymer containng at leasts 30 molar % of a repeating unit selected from those consisting of a repeating unit having a sulfoalkyl group and a repeating unit having a sulfophenyl group, and in which a nondiffusive base containing layer is provided under the condition that the nondiffusive base-containing layer is in liquid contact with said porous reagent layer, and wherein said polymer provides a pH environment which preserves the diazonium salt in the reagent layer.

11. The analytical element of claim 10 in which said nondiffusive base is in the form of a polymer.

12. The analytical element of claim 10 in which the repeating unit is a repeating unit having a sulfophenyl group.

13. The analytical element of claim 10 in which said polymer is a homopolymer consisting essentially of a repeating unit selected from those consisting of a repeating unit having a sulfoalkyl group and a repeating unit having a sulfophenyl group.