Apparatus for registering the presence of bacteria, particularly in field conditions

• US 4,672,039 A
• Filed: 10/04/1985
• Issued: 06/09/1987
• Est. Priority Date: 02/10/1984
• Status: Expired due to Fees

• Current Assignee: Svanebergs Foretagskonsulter AB
• Original Assignee: AB Sangtec Medical
• Inventors: Lundblom, Einar
• Primary Examiner(s): Jones, Larry
• Application Number:
  US06/787,791
• Time in Patent Office: 613 Days
• Field of Search: 435/291, 435/311, 435/808, 422/52
• US Class Current: 435/288.7
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404
• CPC Class Codes: G01N 21/763 Bioluminescence
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus

Abstract

The invention relates to an apparatus for registering the presence of bacteria, particularly under field conditions. The apparatus includes a plate (1), in which there is formed a luminescence chamber (2) which is defined at its opening (3) by a support lattice (5) carrying a filter (4), the lattice opening out into and being carrying sealingly against the plate (1) with the aid of a liquid-sealing and opaque collection vessel (6). The plate (1) includes a transparent portion (7) which is situated above or opposite the opening (3) of the chamber (2). There is a means (8) above the portion (7) for registering a light flux caused by luminescence, said means (8) being accommodated in an opaque housing (13) sealing against the plate (1), and there is an aperture plate (9) displaceably arranged above the portion (7) between the plate (1) and the means (8) to prevent unintentional exposure of the means. Bacteria are enriched from a liquid sample on the filter (4), a chemical treatment of the bacteria on the filter provides light in the chamber by luminescence, the luminescence in the chamber being dependent on the chemicals being retained in the chamber by filter (4). Registration of the light taking place electronically or on a light-sensitive, rapidly developing, photographic film, when the light image which the luminescence achieves is either registered electronically or is read off on the film in the form of a large or smaller light patch or in the form of a grey tone which can be compared with a grey tone scale to define a measure of the bacteria content.

Claims

1. Apparatus of a portable kind for registering the presence of bacteria, particularly under field conditions, comprising;

at least one luminescence chamber formed in a plate made of transparent material;

said luminescence chamber having an opening which communicates with a filter which rests on a perforated support lattice held sealingly against said plate by a water-tight and opaque collection vessel, said luminescence chamber further having a communication duct for injecting a liquid sample and chemicals and a venting duct which passes from said luminescence chamber to said collection vessel, said venting duct being closable with the aid of a valve;

the portion of said plate opposite said opening being transparent such that light flux created by luminescence within said luminscence chamber passes through said transparent portion and is incident onto means for registering said light, said means for registering being situated in an opaque housing sealed against said plate and covering said transparent portion of said plate.

2. Apparatus as claimed in claim 1, wherein the means for registering the light occurring due to luminescence comprises a light-sensitive photographic film.

3. Apparatus as claimed in claim 1 or 2, wherein an aperture plate is displaceably arranged between said portion of said plate and the light sensitive photographic film for preventing unintentional exposure of the film.

4. Apparatus as claimed in claim 1, wherein said means for registering the light occurring due to luminescence comprises an electronic light detector for registering the light generated by the reaction in the chamber.

5. Apparatus as claimed in claim 1, wherein said luminescence chamber is cylindrical in shape, and said communication duct for infecting a liquid sample and chemicals extends through said plate and passes tangentially into said luminescence chamber.