Optical device with a high collection efficiency and cytofluorimeter making use of the same
First Claim
1. An optical device permitting at least one of the two following operations, namely(a) the luminous excitation of an axial flow of material to be analyzed, said flow being directed along an axis and being able to emit light when said flow receives some light and(b) the collection of light emitted by said flow,said device comprising a first optical element and an analysis chamber which chamber has a spherical shape, is formed within the first optical element and has a symmetry centre, said chamber being open at two diametrically opposite ends,the first optical element having an inner concave spherical dioptre, an outer convex spherical dioptre, and an axis of symmetry passing through the symmetry centre of the chamber andthe first optical element being positioned in such a way that the axis of the material flow passes through the two ends and the centre of the chamber,said optical device also comprising a second optical element having an inner concave dioptre, an outer convex dioptre and an axis of symmetry, said second optical element positioned in such a way that the inner dioptre of the second optical element faces the outer dioptre of the first optical element, and that the symmetry axis of the second optical element coincides with the symmetry axis of the first optical element.
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Accused Products
Abstract
Optical device with a high collection efficiency and cytofluorimeter using the same. This device permits the light excitation of a flow of material directed along an axis and able to emit light when it receives it and/or collects the light emitted by said flow. It comprises a first optical element and a spherical analysis chamber which can be penetrated by the flow, defined by the element and allowing a center of symmetry. The element has a convex spherical dioptre allowing an axis of symmetry passing through the center of the chamber and said element is positioned in such a way that the axis of the flow passes through the center of the chamber. Application to the analysis of a flow of biological cells.
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Citations
16 Claims
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1. An optical device permitting at least one of the two following operations, namely
(a) the luminous excitation of an axial flow of material to be analyzed, said flow being directed along an axis and being able to emit light when said flow receives some light and (b) the collection of light emitted by said flow, said device comprising a first optical element and an analysis chamber which chamber has a spherical shape, is formed within the first optical element and has a symmetry centre, said chamber being open at two diametrically opposite ends, the first optical element having an inner concave spherical dioptre, an outer convex spherical dioptre, and an axis of symmetry passing through the symmetry centre of the chamber and the first optical element being positioned in such a way that the axis of the material flow passes through the two ends and the centre of the chamber, said optical device also comprising a second optical element having an inner concave dioptre, an outer convex dioptre and an axis of symmetry, said second optical element positioned in such a way that the inner dioptre of the second optical element faces the outer dioptre of the first optical element, and that the symmetry axis of the second optical element coincides with the symmetry axis of the first optical element.
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7. A cytofluorimeter comprising:
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means for forming a flow of biological cells directed along an axis, said cells being able to emit light when they receive some light; means for forming at least one light beam for causing luminous excitation of said cells; an optical device for transmitting said light beam thereby permitting said cell excitation and the collection of any light emitted by the flow of cells; and means for analyzing said light; wherein the optical device comprises a first optical element and a spherical analysis chamber formed within said first optical element and having a symmetry centre, said chamber being open at two diametrically opposite ends, the first optical element having an inner concave spherical dioptre, an outer convex spherical dioptre, and an axis of symmetry passing through the symmetry centre of the chamber and the first optical element being positioned in such a way that the axis of the material flow passes thorugh the two ends and the centre of the chamber. said optical device also comprises a second optical element having an inner concave dioptre, an outer convex dioptre and an axis of symmetry, said second optical element positioned in such a way that the inner dioptre of the second optical element faces the outer dioptre of the first optical element, and that the symmetry axis of the second optical element coincides with the symmetry axis of the first optical element. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification