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Self-contained reagent package device and an assay using same

  • US 4,675,299 A
  • Filed: 12/12/1984
  • Issued: 06/23/1987
  • Est. Priority Date: 12/12/1984
  • Status: Expired due to Term
First Claim
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1. A self-contained reagent package device useful in the performance of chemical and biological assays comprising:

  • a rack;

    a plurality of wells in said rack, all of said wells having open top ends for access thereto, and all but one of said wells having closed bottom ends, said one well having an aperture at its bottom end for the passage of liquids therethrough, said one well including beads to which a reagent is bound and further including a porous filter positioned between said beads and said aperture which is constructed so as to retain liquids used in the assay under normal atmospheric pressure conditions but permitting the passage of said liquids, including unbound and soluble substances, therethrough at increased pressure within said one well, said filter further being constructed so as to retain the beads and substances bound thereto;

    at least one of said wells with a closed bottom having a predetermined amount of reagent therein;

    another of said wells with a closed bottom being empty so that the specimen to be assayed may be deposited therein; and

    a removable protective cover sealed over the open ends of said wells to maintain the incorporated reagents in stable form prior to use thereby providing a self-contained reagent package device.

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