Automatic random access analyzer

• US 4,678,752 A
• Filed: 11/18/1985
• Issued: 07/07/1987
• Est. Priority Date: 11/18/1985
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Miles, Scott D., Claus, Michael J., Goldberg, Ruth D., Thorne, Gale H., Owen, Charles V., Smith, Randall W.
• Primary Examiner(s): Scott, Samuel
• Assistant Examiner(s): Kamen, Noah
• Application Number:
  US06/799,238
• Time in Patent Office: 596 Days
• Field of Search: 422/63-67, 422/100, 422/102, 435/291
• US Class Current: 435/287.3
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/0093 random access not determined by physical position
• CPC Class Codes: G01N 2035/0465 Loading or unloading the conveyor
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 35/0092 Scheduling
• CPC Class Codes: G01N 35/02 using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations G01N35/0098 and G01N35/0099 take precedence
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes

Abstract

An automated analytical apparatus comprises an introduction station for receiving a plurality of reagent packages. Each package has a plurality of receptacles, at least one of which includes a sample liquid to be analyzed, and at least one of which includes a liquid reagent for forming a reaction mixture with the sample liquid. A liquid transfer station is included for transferring liquid contained in one or more receptacles of a reagent package into, out of or among different receptacles thereof, including the formation of the reaction mixture. A detector is provided for detecting a characteristic of the sample by analyzing the reaction mixture contained in one of the receptacles of a package. A storage area is included within the apparatus for storing a plurality of reagent packages. A shuttle system transports individual reagent packages in any order and in any direction between the introduction area, the liquid transfer station, the detector and the storage area.

Claims

1. An automated apparatus for analysis of samples comprising:

an introduction station for the placement of a rack containing a plurality of reagent packages, each package having a plurality of receptacles at least one of which includes a sample liquid, and at least one of which includes a liquid reagent for forming a reaction mixture with said sample liquid, said introduction station including a platform for holding an elongate rack having a plurality of slots aligned in substantially parallel arrangement along the longitudinal axis of said rack, said reagent packages being removably positioned in said slots;

a liquid transfer station for the transfer of liquid contained in one or more receptacles of a reagent package among the different receptacles of said one reagent package so that a reaction mixture may be formed;

an incubation storage area for holding a plurality of reagent packages which have passed through said liquid transfer station;

a shuttle system for moving said rack so that each package therein is positionable adjacent said liquid transfer station and for removing one of said packages at a time from the rack and moving it into said transfer station and into said incubation storage area, said shuttle system being further operative to move individual packages from the storage area into said transfer station and into said rack in the introduction station; and

a detector for analyzing the reaction mixture contained in one of the receptacles of any of said packages for determining a characteristic of the sample.

2. The apparatus of claim 1 wherein said liquid transfer station includes a dual-action pump for aspirating liquid from one or more receptacles of a single reagent package and for delivering said aspirated liquid into one or more receptacles of said reagent package, whereby said transfer of liquids includes the mixing of said sample liquid and said liquid reagent to form a reaction mixture in at least one of the receptacles of said package.

3. The apparatus of claim 2 wherein said liquid transfer station includes a hollow tube having its lumen in fluid communication with said pump, said tube being operatively mounted in said liquid transfer station to move into and out of the receptacles of the package for the transfer of liquids.

4. The apparatus of claim 3 wherein said liquid transfer station further includes a reservoir in the fluid path between said tube and said pump for the temporary storage of liquid aspirated from one or more of said receptacles.

5. The apparatus of claim 4 wherein said liquid transfer station further includes a heater for heating the liquid in said reservoir.

6. The appparatus of claim 3 wherein said tube includes a liquid level sensor for sensing the level of liquid in any of the receptacles into which the tube is moved.

7. The apparatus of claim 3 wherein said liquid transfer station includes an operative punch member having a sharp point at its distal end for piercing a thin protective cover sealed over open ends of one or more receptacles of the reagent package to thereby gain entry into such receptacles.

8. The apparatus of claim 4 wherein said liquid transfer station further comprises rinsing means in fluid communication with said tube and said reservoir for passing rinsing liquid therethrough for cleaning purposes.

9. The apparatus of claim 1 wherein said liquid transfer station further includes a withdrawal pump for withdrawing waste liquid from one or more receptacles of said package and delivering said waste liquid to a waste collection area.

10. The apparatus of claim 1 which further includes a device for automatically obtaining test-related information from the reagent package prior to the transfer of liquids with respect to the receptacles thereof.

11. The apparatus of claim 10 wherein said device is a bar-code scanner for electro-optically reading a bar-code label on a reagent package, said apparatus further including means for processing said label information obtained by the bar-code scanner for performing the analysis of the sample.

12. The apparatus of claim 11 wherein said processing means includes means for electro-optically reading a set marker associated with said rack for identifying the set according to which the reagent packages are positioned in said rack and for performing the analyses in accordance with said set.

13. The apparatus of claim 12 wherein said processing means controls the order that individual reagent packages, grouped by set, are transported by said shuttle system from the rack to the liquid transfer station.

14. The apparatus of claim 13 wherein said processing means is operative to read more than one set marker on a given rack and to perform different analyses according to the different sets.

15. The apparatus of claim 1 wherein said shuttle system includes a first drive mechanism to incrementally step said rack positioned in said introduction station so that any one of said reagent packages is positionable adjacent said liquid transfer station.

16. The apparatus of claim 15 wherein said drive mechanism is operative to step said rack translationally, in either direction, across the entrance to said liquid transfer station, said shuttle system further including a second drive mechanism operative in a direction substantially perpendicular to said first drive mechanism for removing one reagent package at a time from said rack and transporting said package to said liquid transfer station and said incubation storage area, and in the reverse direction back to said rack.

17. The apparatus of claim 16 wherein said second drive mechanism and said liquid transfer station cooperatively include means for maintaining the reagent package in substantially movement-free position when such package is in said liquid transfer station.

18. The apparatus of claim 17 wherein said maintaining means includes a spring-biased wall engageable with said reagent package for maintaining the position of said package in the liquid transfer station.

19. The apparatus of claim 1 wherein the incubation storage area includes a rotatable carousel having a plurality of radially extending slots for the receipt of said reagent packages transported thereto by said shuttle system.

20. The apparatus of claim 19 wherein said carousel is rotatable in clockwise and counterclockwise directions to either accept from or deliver to said shuttle system the reagent packages.

21. The apparatus of claim 19 wherein rotation of said carousel for accepting or delivering reagent packages is regulated by control means.

22. The apparatus of claim 19 wherein said carousel is in a temperature-controlled environment for incubating the reagent packages.

23. The apparatus of claim 22 wherein said environment is an air bath maintainable at a relatively constant temperature.

24. The apparatus of claim 1 which further includes means for positioning individual reagent packages adjacent to said detector so that one such package at a time may be analyzed thereby.

25. The apparatus of claim 24 wherein said detector includes a light source for directing light energy into one of the receptacles of the reagent package which contains the reaction mixture for analysis.

26. The apparatus of claim 25 wherein said detector includes at least one means for receiving light associated with the receptacle into which light energy is directed and for associating said received light with a characteristic of the sample under analysis.

27. The apparatus of claim 1 which further includes assay formatting means associated with said liquid transfer station for regulating the actions of said liquid transfer station for transferring liquid into, out of or among the receptacles of a reagent package.

28. The apparatus of claim 27 wherein said formatting means includes one or more control mechanisms for recognizing the type of liquids contained in each package, the location of such liquids, the volumes of liquid in the receptacles and the transfer requirements into, out of or among the receptacles.

29. The apparatus of claim 28 wherein said control mechanisms include a computer program into which information regarding said liquids is storable, retrievable, usable or changeable for analysis of the sample.

30. The apparatus of claim 1 which further includes scheduling means for determining the sequence that reagent packages pass, in any order and in any direction, between the rack, the liquid transfer station, the incubation storage area and the detector.

31. The apparatus of claim 30 wherein said scheduling means includes means for determining when reagent packages are introduced into the apparatus, for recalling information about reagent packages previously introduced into the apparatus and for scheduling a sequence for processing the identified reagent packages according to pre-programmed priorities.

32. The apparatus of claim 31 wherein said scheduling means is pre-programmed to schedule processing of reagent packages in the following order or priority:

a. reagent packages on which processing has started to be continued;

b. specially designated reagent packages for immediate processing;

c. reagent packages which have been in the apparatus longer than a pre-determined time and on which processing has not begun; and

d. reagent packages recently introduced into the apparatus.

33. The apparatus of claim 32 wherein reagent packages of more complexity within each listed scheduling category are scheduled with a higher priority, complexity being determined by the number of processing steps to process any reagent package.

34. The apparatus of claim 33 wherein said scheduling means accords a higher priority to a reagent package, within each listed scheduling category, which has a shorter incubation period than other packages in the same scheduling group.

35. The apparatus of claim 34 wherein said scheduling means includes a computer program into which information regarding said scheduling is storable, retrievable, usable or changeable for analysis of the samples.

36. An automated analytical apparatus comprising:

means for receiving a plurality of reagent packages each having a plurality of receptacles at least one of which includes a sample liquid, and at least one of which includes a liquid reagent for forming a reaction mixture with said sample liquid, said means for receiving including a platform for holding an elongate rack having a plurality of slots along a longitudinal axis thereof, said reagent packages being removably positioned in said slots;

means for transferring liquid contained in one or more receptacles of a reagent package to a different receptacle thereof, whereby a reaction mixture may be formed;

detector means for detecting a characteristic of the sample by analyzing the reaction mixture contained in one of the receptacles of a package;

means for storing a plurality of reagent packages; and

means for transporting individual reagent packages in any order and in any direction between the means for receiving, the means for transferring liquid, the detector means and the means for storing.

37. An automated apparatus for analysis of samples comprising:

an introduction station for the placement of a rack containing a plurality of reagent packages, each package having a plurality of receptacles at least one of which includes a sample liquid, and at least one of which includes a liquid reagent for forming a reaction mixture with said sample liquid;

a liquid transfer station including a dual action pump for aspirating liquid from one or more receptacles of a single reagent package and for delivering said aspirated liquid into one or more receptacles of said reagent package, said transfer of liquids including the mixing of said sample liquid and said liquid reagent to form a reaction mixture in at least one of the receptacles of said package;

a device for automatically obtaining test related information from the reagent package prior to the transfer of liquids with respect to the receptacles thereof;

means for processing said obtained information for performing the analysis of the reaction mixture, said processing means including means for reading a set marker associated with said rack for identifying the set according to which the reagent packages are positioned in said rack and for performing the analyses in accordance with said set, and further including assay formatting means associated with said liquid transfer station for regulating the actions of said liquid transfer station for transferring liquid into, out of or among the receptacles of the reagent package;

an incubation storage area including a rotatable carousel having a plurality of radially extending slots for the receipt of said reagent packages transported thereto, said carousel being in a temperature-controlled environment for incubating the reagent packages;

a shuttle system for moving said rack so that each package therein is positionable adjacent said liquid transfer station and for removing one of said packages at a time from the rack and moving it into said transfer station and into said carousel, said shuttle system being further operative to move individual packages from the carousel into said transfer station and into said rack in the introduction station;

a detector including a light source for directing light energy into one of the receptacles of the reagent package which contains the reaction mixture for analysis and further including means for receiving light associated with the receptacle into which light energy is directed and for associating said received light with a characteristic of the sample under analysis; and

scheduling means for determining the sequence that reagent packages pass, in any order and in any direction, between the rack, the liquid transfer station, the carousel and the detector.