Glucose sensor
First Claim
1. Glucose sensor means for in-vivo generation of an electrical signal in response to the presence of glucose in the bloodstream and thereby electrically determining blood glucose concentrations, said sensor means comprising an iridium substrate with an in-situ oxidized outer surface of iridium oxide to which are bonded first and second superposed films, said first film being bonded to said iridium oxide surface and consisting essentially of silanized gamma aminopropyltriethoxysilane, and with said second film consisting essentially of glucose oxidase and being covalently bonded in-situ onto said first film.
0 Assignments
0 Petitions
Accused Products
Abstract
A glucose sensor for electrically determining blood glucose concentration for in vivo and in vitro applications and comprising an iridium substrate with an oxidized surface, to which is bonded a first film of silanized gamma propyltriethoxysilane and a second film of glucose oxidase covalently bonded thereto. The electrode provides an electrial signal, the amplitude of which varies in response to changes in blood glucose concentration, with the electrode being both rugged and stable.
552 Citations
6 Claims
-
1. Glucose sensor means for in-vivo generation of an electrical signal in response to the presence of glucose in the bloodstream and thereby electrically determining blood glucose concentrations, said sensor means comprising an iridium substrate with an in-situ oxidized outer surface of iridium oxide to which are bonded first and second superposed films, said first film being bonded to said iridium oxide surface and consisting essentially of silanized gamma aminopropyltriethoxysilane, and with said second film consisting essentially of glucose oxidase and being covalently bonded in-situ onto said first film.
-
2. Glucose sensor means for in-vivo generation of an electrical signal in response to the presence of glucose in the bloodstream and thereby electrically determining blood glucose concentrations and comprising an iridium base substrate having an in-situ coating of iridium oxide on the surface thereof, and with a first film adherently bonded thereto and a second film adherently and serially bonded onto the surface of said first film, said first film consisting essentially of a silanized layer of gamma aminopropyltriethoxysilane and said second film consisting of essentially glucose oxidase covalently bonded to the surface of said first film, and with a silicone rubber film being applied to the outer surface of said glucose oxidase film.
-
3. The method of preparing a glucose sensor for electrically determining blood glucose concentrations including the steps of:
-
(1) oxidizing the surface of an iridium base to form a layer of in-situ oxide thereon; (2) thereafter exposing the iridium oxide surface to a solution of gamma aminopropyltriethoxysilane to prepare a silanized film thereon; (3) thereafter treating the silanized coating with an acqueous solution of glucose oxidase to form a covalently bonded film of glucose oxidase on the silanized film; and (4) thereafter treating the glucose oxidase film through application of a film of silicone rubber to the surface thereof. - View Dependent Claims (4, 5, 6)
-
Specification