Composition for stimulating bone growth

• US 4,681,763 A
• Filed: 06/11/1985
• Issued: 07/21/1987
• Est. Priority Date: 06/11/1985
• Status: Expired due to Fees

• Current Assignee: University of Medicine and Dentistry of New Jersey 100 Bergen Street NJ
• Original Assignee: University of Medicine and Dentistry of New Jersey (State of New Jersey)
• Inventors: Sampath, T. K., Nathanson, Mark A., Reddi, A. H.
• Primary Examiner(s): Brown, Johnnie R.
• Assistant Examiner(s): Rollins, John W.
• Application Number:
  US06/743,598
• Time in Patent Office: 770 Days
• Field of Search: 424/95
• US Class Current: 424/426
• CPC Class Codes: A61K 35/32 Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane

Abstract

There is disclosed a method of recovering a material active in the formation of new bone and cartilage from demineralized bone extract and the composition of said material on a pliable physiologically acceptable support.

Claims

1. A composition for implantation into animals to stimulate new bone growth comprising:

a material capable of stimulating formation of new bone and cartilage in vivo derived from demineralized bone by use of aqueous guanidine hydrochloride, said material deposited by precipitation onto a pliable physiologically acceptable support.

2. The composition of claim 1, wherein the pliable physiologically acceptable support comprises collagen gel.

3. A method of recovering a material capable of stimulating cartilage in vivo derived from demineralized bone by use of aqueous guanidine hdrochloride comprising:

(a) applying a solution of said material in aqueous guanidine hydrochloride to a pliable physiologically acceptable support;

(b) precipitating said material from the solution of aqueous guanidine hydrochloride onto the support by the addition of a precipitating agent; and

(c) removing the volatile alcohol solvent.

4. The method of claim 3, wherein the precipitating agent is a volatile alcohol solvent.

5. The method of claim 3, wherein the temperature of the precipitating agent is -10°

C.

6. The method of claim 3, wherein the concentration of the precipitating agent is 85%.

7. A method of preparing an implant to induce new bone growth which comprises

(a) providing a solution of a material capable of stimulating formation of new bone and cartilage in vivo derived from demineralized bone by use of aqueous guanidine hydrochloride;

(b) applying said solution to a pliable physiologically acceptable support; and

(c) percipitating said material from said solution onto said support.

8. The method of claim 7 wherein the pliable physiologically acceptable support is a collagen gel.

9. The method of claim 7 wherein the precipitation of said material is caused by the addition of an alcohol solvent.

10. The method of claim 9 wherein the alcohol solvent is ethanol or isopropanol.

11. A method of stimulating formation of new bone and cartilage in vivo which comprises implanting in a patient in need of such stimulation therapy an implant which comprises a material capable of stimulating formation of new bone and cartilage in vivo derived from demineralized bone by use of aqueous guanidine hydrochloride said material deposited by precipitation onto a pliable physiologically acceptable support.

12. The method of claim 11 wherein the physiologically acceptable support is a collagen gel.