×

Protein A-silica immunoadsorbent and process for its production

  • US 4,681,870 A
  • Filed: 01/11/1985
  • Issued: 07/21/1987
  • Est. Priority Date: 01/11/1985
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for preparing an immunoadsorbent material useful for removing IgG and IgG-complexes from biological fluids, said method comprising:

  • introducing free amino or carboxyl groups onto a silica matrix;

    reacting the silica matrix with purified protein A in the presence of a carbodiimide at a pH in the range from 3.5 to 4.5 to covalently link the protein A to the matrix through the amino or carboxyl groups; and

    washing the silica matrix at a pH in the range from 2.0 to 2.5 to remove loosely bound protein A from the matrix.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×