Encapsulated mouse cells transformed with avian retrovirus-bovine growth hormone DNA, and a method of administering BGH in vivo

• US 4,686,098 A
• Filed: 05/14/1984
• Issued: 08/11/1987
• Est. Priority Date: 05/14/1984
• Status: Expired due to Fees

• Current Assignee: Merck & Co., Inc.
• Original Assignee: Merck & Co., Inc.
• Inventors: Kopchick, John J., Leung, Frederick C., Livelli, Thomas J., Malavarca, Richard H.
• Primary Examiner(s): GRIFFIN, RONALD
• Application Number:
  US06/609,924
• Time in Patent Office: 1,184 Days
• Field of Search: 435/177, 435/182, 424/33, 424/15, 514/12, 604/891
• US Class Current: 424/424
• CPC Class Codes: A01K 67/0271 Chimeric vertebrates, e.g. comprising exogenous cells
• CPC Class Codes: A61K 38/27 Growth hormone [GH], i.e. somatotropin
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: A61K 9/0092 Hollow drug-filled fibres, tubes of the core-shell type, coated fibres, coated rods, microtubules or nanotubes
• CPC Class Codes: C07K 14/61 Growth hormone [GH], i.e. somatotropin
• CPC Class Codes: C12N 11/04 entrapped within the carrier, e.g. gel or hollow fibres

Abstract

Recombinant DNA constructs having an avian retroviral long terminal repeat (LTR) ligated to the bovine growth hormone gene, were co-transformed into a mammalian cell (mouse) culture in order to obtain a stable cell culture secreting large amounts of bovine growth hormone. The transformed mouse cells were encapsulated in hollow fibers and implanted into animals, thereby producing circulating bovine growth hormone.

Claims

1. The method for increasing animal growth by implantation in vivo in cows an effective amount of the encapsulated cell line L-BGH-4-3, ATCC CRL 8537 or L-BGH-4-13, ATCC CRL-8536.

2. The method of claim 1 in which the encapsulated cell line is implanted subcutaneously.

3. A hollow fiber, about 10 mm long polyvinyl chloride-acrylic copolymer, having about a 1100 μ

m internal diameter, containing a recombinant mouse cell suspension capable of producing bovine growth hormone in an amount sufficient to stimulate animal growth or milk production when implanted in an animal.