Composition and sampling receptacle method for treating blood with anticoagulant

• US 4,687,000 A
• Filed: 03/15/1985
• Issued: 08/18/1987
• Est. Priority Date: 07/15/1983
• Status: Expired due to Term

• Current Assignee: Radiometer A/S (Danaher Corporation)
• Original Assignee: Radiometer A/S (Danaher Corporation)
• Inventors: Hansen, Ole M., Eisenhardt, Anne R.
• Primary Examiner(s): Howell, Kyle L.
• Assistant Examiner(s): SHAY, RANDY CITRIN
• Application Number:
  US06/713,911
• Time in Patent Office: 886 Days
• Field of Search: 128/760, 128/763, 604/269, 604/416
• US Class Current: 600/573
• CPC Class Codes: A61B 5/15003 for venous or arterial blood
• CPC Class Codes: A61B 5/150213 Venting means
• CPC Class Codes: A61B 5/150236 Pistons, i.e. cylindrical bodies that sit inside the syringe barrel, typically with an air tight seal, and slide in the barrel to create a vacuum or to expel blood
• CPC Class Codes: A61B 5/150389 Hollow piercing elements, e.g. canulas, needles, for piercing the skin
• CPC Class Codes: A61B 5/150503 Single-ended needles hollow piercing element tip design A61B5/150396, needle sets for catheters A61M25/06, needles for bringing media into the body A61M5/32
• CPC Class Codes: A61B 5/150587 by friction fit
• CPC Class Codes: A61B 5/150717 manually removed
• CPC Class Codes: A61B 5/153 Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02

Abstract

In order to counteract coagulation of a blood sample which is collected from an animal or a human body (24) and introduced into a receptacle, such as a syringe chamber (14), an anticoagulant is added to the sample within the receptacle. The anticoagulant may be capable of binding cation species in the blood, which are subsequently to be measured. Such cation species comprise hydrogen ions, ammonium ions, alkaline metal ions such as potassium and sodium ions, and alkaline earth metal ions such as magnesium and calcium ions. In order to compensate for the proportions of selected cation species which are bound by the anticoagulant, an additive containing such cation species is added to the blood sample so as to obtain substantially the same concentration of the selected cation species in an unbound condition in the anticoagulant treated blood sample as in the untreated blood sample.

The anticoagulant and the additive are preferably added to the blood sample as a composition which may be deposited on or carried by a carrier body (15) which may be arranged within the receptacle in which the blood sample is introduced. The bulk density of the nonwetted carrier body is preferably smaller than the specific weight of the blood sample so as to avoid trapping of air bubbles within the receptacle. In its wetted condition the bulk density of the carrier body is preferably also different from the specific weight of the blood sample so that the carrier body (15) may be used as an agitating member improving mixing of the anticoagulant composition with the blood sample. The carrier body (15) may, for example, be made of filtering paper.

Claims

1. A blood sampling device comprising receptacle means defining a blood receiving space therein for receiving a blood sample, said means including

a blood inlet means for introducing a sample of whole blood into the blood receiving space,

an anticoagulant present within said space, the anticoagulant being an anticoagulant capable of binding cation ion species in blood, and

an additive present within said space and including several selected cation species which occur in blood, each species in the additive being present in an amount substantially corresponding to the binding capacity for that species that the anticoagulant exhibits when in equilibrium with a blood sample of substantially the same volume as that of said receptacle so as to ensure substantially the same concentration of each of the selected cation species in an unbound condition in the blood after the blood has been introduced into the blood receiving space as is in the blood prior to being introduced into said space.

2. A blood sampling device according to claim 1 wherein the anticoagulant, the additive, or the anticoagulant and the additive are carried by a carrier body.

3. A blood sampling device according to claim 2 wherein the bulk density of the carrier body in a non-wetted state is less than the specific weight of the blood sample.

4. A blood sampling device according to claim 3 wherein the carrier body comprises a porous or fibrous material.

5. A blood sampling device according to claim 4 wherein the carrier body comprises cellulose material.

6. A blood sampling device according to claim 5 wherein the carrier body comprises filtering paper.

7. A blood sampling device according to claim 2 wherein the carrier body has a disc-like shape.

8. A blood sampling device according to claim 1 wherein said selected cation species are selected from the group consisting of hydrogen ions, ammonium ions, alkali metal ions, and alkaline earth metal ions.

9. A blood sampling device according to claim 8 wherein said selected cation species comprise alkali metal ions selected from the group consisting of potassium and sodium ions.

10. A blood sampling device according to claim 8 wherein said selected cation species comprise alkaline earth metal ions selected from the group consisting of magnesium and calcium ions.

11. A blood sampling device according to claim 1 wherein the anticoagulant is selected from the group consisting of heparin and heparin derivatives.

12. A blood sampling device according to claim 11 wherein heparin is present in an amount of from 40 to 100 IU/ml of the blood receiving space.

13. A blood sampling device according to claim 12 wherein heparin is present in an amount of from 50 to 80 IU/ml of the blood receiving space.

14. A blood sampling device according to claim 1 wherein at least part of the additive is combined with the anticoagulant into an anticoagulant composition.

15. A blood sampling device according to claim 10 wherein at least part of the additive forms a compound with the anticoagulant.

16. A blood sampling device according to claim 15 wherein said compound is a heparin salt.

17. A blood sampling device according to claim 15 wherein said compound is selected from the group consisting of lithium heparinate, sodium heparinate, and potassium heparinate.

18. A blood sampling device according to claim 14 wherein the anticoagulant composition further comprises a salt selected from the group consisting of sodium, potassium, and calcium salts.

19. A blood sampling device according to claim 18 wherein the anticoagulant composition comprises a mixture of lithium heparinate and sodium heparinate in a ratio expressed in International Units of from 65:

35 to 35;

65 as well as a potassium salt.

20. A blood sampling device according to claim 19 wherein the anticoagulant composition comprises a mixture of lithium heparinate and sodium heparinate in a ratio of 50:

50 IU as well as a potassium salt.

21. A blood sampling device according to claim 1 wherein the anticoagulant contains lithium as an inactive cation.

22. A blood sampling device according to claim 1 wherein te anticoagulant is solid.

23. A blood sampling device according to claim 1 wherein the receptacle means is in the form of a syringe comprising a syringe cylinder and a piston displaceably arranged therein.

24. A method of treating a blood sample with an anticoagulant, said method comprising

passing a predetermined volume of a sample of whole blood into a receptacle,

adding to the blood sample within the receptacle a predetermined amount of the anticoagulant, which is capable of binding different cation species in the blood, and

adding to the blood sample within the receptacle an additive including selected ones of said different cation species in amounts compensating for proportions of the selected cation species bound by the anticoagulant so as to obtain substantially the same concentration of the selected cation species in an unbound condition in the anticoagulant treated blood sample as in the untreated blood sample.