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Process for the simultaneous synthesis of several oligonucleotides on a solid phase

  • US 4,689,405 A
  • Filed: 01/04/1984
  • Issued: 08/25/1987
  • Est. Priority Date: 01/20/1983
  • Status: Expired due to Term
First Claim
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1. A process for the simultaneous synthesis of a plurality of different oligonucleotides of predetermined nucleotide sequences on a solid phase, wherein the oligonucleotides are synthesized from mononucleotides, which comprises;

  • (a) providing from one to four groups of solid phase supports in the form of segments of flat material, each group of supports comprising at least one support, and the supports are linked to starting mononucleotide building blocks, which differ from group to group;

    (b) providing from one to four reaction media, each containing a different mononucleotide building block;

    (c) contacting the reaction media sequentially with the supports grouped in accordance with the predetermined sequences of the oligonucleotides(d) where according to the method of synthesis chosen, a deprotection step may precede stage (c) above and a blocking step may follow stage (c) above.

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