Process and device for carrying out analytical determinations

• US 4,690,899 A
• Filed: 07/02/1985
• Issued: 09/01/1987
• Est. Priority Date: 07/06/1984
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Kleemann, Wolfgang, Vieth, Friedhelm, Pasch, Manfred, Schlumberger, Helmut, Klose, Sigmar
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Delahunty, C. M.
• Application Number:
  US06/751,368
• Time in Patent Office: 791 Days
• Field of Search: 422/58, 422/64, 422/72, 422/102, 436/45, 436/165, 436/169, 436/178
• US Class Current: 436/45
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/563 involving antibody fragments
• CPC Class Codes: G01N 33/6857 Antibody fragments
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor

Abstract

In a process for carrying out an analytical determination by mixing and incubating a sample liquid with at least one dry reagent which is soluble therein to form a reaction mixture liquid and measuring a parameter in the reaction mixture liquid, the sample liquid is first transported along a transport path to the soluble dry reagent and the resulting reaction mixture liquid then further transported along the transport path. The further transporting of the reaction mixture liquid along the transport path is under the influence of a force-change cycle in which centrifugal force and a further force alternately predominate to determine the direction of the further transporting along the transport path. At least one further liquid is transported on another path, part of which is separate from and part of which is common with the transport path, by the force-change cycle in such a manner that at least two of the liquids pass through the common part of the transport path chronologically separated.

Claims

1. In a process for carrying out an analytical determination by mixing and incubating a sample liquid with at least one dry reagent soluble therein to form a reaction mixture liquid and measuring a parameter in the reaction mixture liquid, the improvement comprising:

transporting a sample liquid along a first transport path to a dry reagent positioned in said first transport path and which is soluble in said sample liquid for at least partially dissolving of the latter in the sample liquid to form a reaction mixture liquid;

then further transporting the reaction mixture liquid along the first transport path under the influence of a force-change cycle in which centrifugal force and a further force alternately predominate to determine the direction of the further transporting along the first transport path; and

transporting at least one further liquid along a second path, an initial part of which is separate from and a downstream part of which is common with the first transport path, by the force-change cycle simultaneously with the further transporting of the reaction mixture liquid in such a manner that at least the reaction mixture liquid and one of said further liquids pass through the common part of the transport path chronologically separated.

2. Process according to claim 1, and further comprising having at least one insoluble material in the common part of the first and second transport paths which reacts with the reaction mixture liquid and, differently, with the further liquid, the two reactions taking place chronologically separated from one another by the chronological separation of the transporting of the liquids along the common part of the first and second transport paths, the reaction mixture liquid reaching the common part of the first and second transport paths and, thus, the insoluble material therein for its reaction therewith first, and the further liquid thereafter.

3. Process according to claim 2, wherein the soluble, dry reagent is a marked, immunologically-active material which is bindable with a substance to be determined and the insoluble reactive material is a material immunologically bindable with the marked, immunologically-active material.

4. Process according to claim 1, wherein at least one of the first and second transport paths is so constructed that the liquid transported therein passes only a portion of that part of said at least one of the first and second transport paths which is not common to both said first and second transport paths during each force-change cycle.

5. Process according to claim 4 wherein in both said first and second transport paths only a portion of that part of each of said first and second transport paths which is not common to both said first and second transport paths in passed by its respective liquid during each force change cycle and wherein one of said first and second transport paths requires more force change cycles to move its respective liquid from an initial portion of said one of said first and second transport paths to said common part of said first and second transport paths than is required by the other of said first and second transport paths.

6. Process according to claim 5, and further comprising having at least one insoluble material in the common part of the first and second transport paths which reacts with the reaction mixture liquid and, differently, with the further liquid, the two reactions taking place chronologically separated from one another by the chronological separation of the transporting of the liquids along the common part of the first and second transport paths, the reaction mixture liquid reaching the common part of the first and second transport paths and, thus, the insoluble material therein for its reaction therewith first, and the further liquid thereafter.

7. Process according to claim 6, wherein the soluble, dry reagent is a marked, immunologically-active material which is bindable with a substance to be determined and the insoluble reactive material is a material immunologically bindable with the marked, immunologically-active material.

8. In a process for carrying out an analytical determination by mixing and incubating a sample liquid with at least one dry reagent soluble therein to form a reaction mixture liquid, the improvement comprising:

transporting a sample liquid along a first transport path to a dry reagent positioned in said first transport path and which is soluble in said sample liquid for at least partially dissolving of the latter in the sample liquid to form a reaction mixture liquid;

then further transporting the reaction mixture liquid along the first transport path under the influence of a force-change cycle in which centrifugal force and a further force alternatively predominate to determine the direction of the further transporting along the first transport path; and

transporting at least two further liquids along a second and a third path, an initial part of each of said second and third paths being separate from and a downstream part of each of said second and third paths being common with the first transport path, by the force-change cycle simultaneously with the further transporting of the reaction mixture liquid in such a manner that the reaction mixture liquid and at least one of said at least two further liquids pass through the common part of the transport path chronologically separated.