Radioactive labeling of proteins with nucleosides or nucleotides

• US 4,692,509 A
• Filed: 11/27/1984
• Issued: 09/08/1987
• Est. Priority Date: 11/27/1984
• Status: Expired due to Fees

• Current Assignee: Molecular Diagnostics, Inc. (Medite Cancer Diagnostics, Inc.)
• Original Assignee: Molecular Diagnostics, Inc. (Medite Cancer Diagnostics, Inc.)
• Inventors: Dattagupta, Nanibhushan
• Primary Examiner(s): Schain, Howard E.
• Application Number:
  US06/675,373
• Time in Patent Office: 1,015 Days
• Field of Search: 260/112 R, 260/112 B, 424/1.1, 435/6, 435/29, 435/34, 536/27, 422/61, 530/303, 530/380, 530/389, 530/395, 530/406, 530/410, 530/411
• US Class Current: 530/303
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: G01N 33/534 with radioactive label
• CPC Class Codes: G01N 33/564 for pre-existing immune complex or autoimmune disease , i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/828 Protein A
• CPC Class Codes: Y10S 530/868 involving autoimmunity, allergy, immediate hypersensitivity, delayed hypersensitivity, immunosuppression, or immunotolerance

Abstract

A radioactively labeled protein comprising a protein, and a radioactive nucleoside or nucleotide, the protein being covalently linked to the nucleoside or nucleotide. Advantageously the linkage is through an NH₂ group of the protein and through a carbonyl group of a ring-opened sugar moiety of the nucleoside or nucleotide. The protein can be insulin, an immunoglobulin or protein A. The radioactive moiety may be a P, C, S, H, I or Hg atom. The labels can be used to indicate the presence and amount of the protein in a biological assay.

Claims

1. A radioactively labeled protein comprising a protein, and a radioactive nucloeside or nucleotide, wherein a radioactive moiety is present in the nucleoside or nucleotide, the protein being convalently linked to the nucleoside or nucleotide.

2. A radioactively labeled protein according to claim 1, wherein linkage is through an NH₂ group of the protein.

3. A radioactively labeled protein according to claim 2, wherein the linkage is through a carbonyl group of a ring-opened sugar moiety of the nucloeside or nucleotide.

4. A radioactively labeled protein according to claim 1, wherein the radioactive nucleoside or nucleotide carries a radioactive phosphorus atom.

5. A radioactively labeled protein according to claim 1, wherein the radioactive nucleoside or nucleotide carries a radioactive carbon atom.

6. A radioactively labeled protein according to to claim 1, wherein the radioactive nucleoside or nucleotide carries a radioactive iodine hydrogen, sulfur or atom.

7. A radioactively labeled protein according to claim 1, wherein the radioactive nucleoside or nucleotide carries a radioactive mercury atom.

8. A radioactively labeled protein according to claim 1, wherein the protein comprises insulin.

9. A radioactively labeled protein according to claim 1, wherein the protein comprises an immunoglobulin.

10. A radioactively labeled protein according to claim 1, wherein the protein comprises protein A.

11. A radioactively labeled protein according to claim 1, wherein the nucleotide is ATP.

12. A radioactively labeled protein according to claim 1, wherein the protein is linked to a nucleoside.