Electrical measuring apparatus, and methods for determining the condition or identity of biological material

• US 4,692,685 A
• Filed: 03/14/1985
• Issued: 09/08/1987
• Est. Priority Date: 03/14/1984
• Status: Expired due to Fees

• Current Assignee: Kevin L. Blaze
• Original Assignee: Kevin L. Blaze
• Inventors: Blaze, Kevin L.
• Primary Examiner(s): Scott, Samuel
• Assistant Examiner(s): KAMEN, NOAH P
• Application Number:
  US06/711,795
• Time in Patent Office: 908 Days
• Field of Search: 324/61 R, 324/65 R, 324/61 QS, 435/291
• US Class Current: 324/692
• CPC Class Codes: G01N 33/4833 of solid biological material, e.g. tissue samples, cell cultures tissue in vivo A61B5/00; cell suspensions G01N33/48735

Abstract

This invention relates to a measurement system which applies voltage signals of different frequencies across a biological sample. The frequencies are chosen such that both resistance and capacitance are significant components of the measured impedance. The state of the biological sample is reflected in the admittance ratio for two frequencies.

Claims

1. Apparatus for determining a measure of the admittance of biological tissue and an admittance ratio for the tissue, for assessing the condition or identity of the tissue, comprising:

A pair of electrical conductors connectable to the respective contacts of a probe having a fixed spacing, between which contacts tissue is disposed in use of the apparatus;

means to generate a constant AC voltage at each of two distinct frequencies at which both the resistance and the capacitance of the tissue between said contacts are significant components of the impedance therebetween;

means to apply said constant AC voltage across said contacts first at one of said frequencies, then at the other;

precision voltage stabilization means coupled to said generation means to maintain the amplitude of said AC voltage at the two frequencies at the same accurately constant value;

buffer means in series with and between said voltage generation means and one of said conductors to minimize the loading effect of the impedance of the tissue on the impedance of the generating means;

amplifier means coupled to the other said conductor, in series with tissue when disposed between said contacts, to receive the current through the tissue and to generate an output AC voltage signal directly proportional to the current through the tissue and therefore to the admittance of the tissue on application of said AC voltage across a portion of the circuit which includes the tissue and the amplifier means; and

means to convert said output AC voltage signal to a DC output which is a measure of the admittance of the tissue between the probe contacts, the admittance ratio, for the two frequencies being thereby determinable from the respective DC outputs at the two frequencies.

2. Apparatus according to claim 1 wherein said amplifier means comprises an operational amplifier with its inputs coupled in series with said other conductor.

3. Apparatus according to claim 1 further including a display coupled to said conversion means to receive the DC output and to display an indication of its value.

4. Apparatus according to claim 1 further including a probe coupled to said electrical conductors, said probe comprising an insulating support body and a pair of parallel needle contacts having a fixed spacing therebetween projecting from said body, the respective electrodes being separately shielded and electrically connected to the respective said conductors and being capable of penetrating biological tissue.

5. Apparatus according to claim 1 wherein said means to maintain said AC voltage constant includes a precision voltage regulator in an emf supply line for said generation means.

6. Apparatus according to claim 1 wherein said AC voltage is no greater than 10 mV rms, in order to minimize errors and irreversible damage to biological tissue caused by power dissipation in the tissue.

7. Apparatus according to claim 1 further including means coupled to said conversion means to receive said DC outputs at the respective frequencies, to derive therefrom said impedance ratio and to output an indication of the value of the ratio and therefore of the condition of biological tissue between said contacts.

8. A method of determining a measure of the admittance of biological tissue and admittance ratio for the tissue, for assessing the condition or identity of the tissue, comprising:

applying an AC voltage maintained at accurately constant amplitude by precision voltage stabilization means across the sample at one frequency than the other, at which frequencies both the resistance and the capacitance of the sample are significant components of the impedance therebetween;

directly measuring the respective currents across the sample by amplifier converter means having a virtual ground configuration in series with the tissue to receive the current through the tissue, and outputting AC voltage signals directly proportional to the respective currents and therefore to the admittance of the tissue;

converting each said AC voltage signal to a DC output, which is a measure of the admittance across the sample, and determining the admittance ratio for the two frequencies from said DC outputs.

9. A method according to claim 8 further comprising determining the admittance ratio for the two frequencies from said DC outputs.

10. a method according to claim 8 wherein said AC voltage is no greater than 10 mV rms, in order to minimize errors and irreversible damage to biological tissue caused by power dissipation in the tissue.