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Device for measuring fluorescence polarization

  • US 4,699,512 A
  • Filed: 07/15/1985
  • Issued: 10/13/1987
  • Est. Priority Date: 07/13/1984
  • Status: Expired due to Term
First Claim
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1. Device for measuring fluorescence polarization comprising:

  • a light source for exciting a sample;

    a spectroscope on the excitation side for separating light emerging from said light source into its spectral components;

    a polarizer for polarizing exciting light coming from said spectroscope on the excitation side;

    an analyzer for selecting a polarization of fluorescence produced by the sample excited by the exciting light coming from said polarizer;

    a spectroscope on the fluorescence side for separating fluorescence light coming from said analyzer into its spectral components;

    a detection system for measuring the intensity of fluorescence light coming from said spectroscope on the fluorescence side;

    a control system permitting to set arbitrarily the polarization angle of said polarizer and that of said analyzer; and

    an arithmetic unit for calculating the degree of fluorescence polarization on the basis of the output of said detection system;

    whereby the polarization angle of the polarizer or the analyzer is successively varied to arbitrary values between 0 and π

    /2 so as to obtain polarization angles and fluorescence intensities corresponding thereto so that the degree of fluorescence polarization is obtained by using a plurality of the polarization angles and the fluorescence intensities corresponding thereto.

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