Electrochemical determination of hematocrit
First Claim
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1. A method for measuring the hematocrit of blood, Hct, comprising:
- (1) measuring concentration of a preselected electrochemically detectable marker, (Marker)WB, naturally present in a preselected volume of whole blood having an initial ratio of red cell volume to plasma volume, the concentration of marker naturally present within red cells being different from its concentration in plasma;
(2) decreasing the ratio of red cell volume to plasma volume by lysing red cells in the preselected volume of whole blood;
(3) measuring the marker concentration after lysing, (Marker)WB; and
(4) calculating the hematocrit using an equation comprising the term;
##EQU4## where;
Hct is hematocrit;
(Marker)WB is measured marker concentration after Lysis;
(Marker)WB is measured marker concentration before Lysis.
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Abstract
A method for measuring hematocrit is provided. An electrochemically detectable marker is measured before and after the ratio of red cell volume to plasma volume is decreased. The measured concentrations are used in a mathematical formula to determine hematocrit value.
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Citations
4 Claims
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1. A method for measuring the hematocrit of blood, Hct, comprising:
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(1) measuring concentration of a preselected electrochemically detectable marker, (Marker)WB, naturally present in a preselected volume of whole blood having an initial ratio of red cell volume to plasma volume, the concentration of marker naturally present within red cells being different from its concentration in plasma; (2) decreasing the ratio of red cell volume to plasma volume by lysing red cells in the preselected volume of whole blood; (3) measuring the marker concentration after lysing, (Marker)WB; and (4) calculating the hematocrit using an equation comprising the term;
##EQU4## where;
Hct is hematocrit;(Marker)WB is measured marker concentration after Lysis; (Marker)WB is measured marker concentration before Lysis. - View Dependent Claims (2, 3, 4)
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Specification