Electrochemical determination of hematocrit

• US 4,699,887 A
• Filed: 03/24/1986
• Issued: 10/13/1987
• Est. Priority Date: 10/04/1984
• Status: Expired due to Term

• Current Assignee: Dade Behring, Inc. (Siemens AG)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Abbott, Scot D., Kobos, Robert K., Peterson, Dale R., Levin, Herman W., Kilkson, Henn
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Cody, Lori-Ann
• Application Number:
  US06/814,404
• Time in Patent Office: 568 Days
• Field of Search: 436/70, 436/63, 436/56, 73/61 R, 73/149, 73/61.4, 422/73
• US Class Current: 436/70
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80
• CPC Class Codes: G01N 33/86 involving blood coagulating time or factors, or their receptors
• CPC Class Codes: Y10T 436/13 Tracers or tags

Abstract

A method for measuring hematocrit is provided. An electrochemically detectable marker is measured before and after the ratio of red cell volume to plasma volume is decreased. The measured concentrations are used in a mathematical formula to determine hematocrit value.

Claims

1. A method for measuring the hematocrit of blood, Hct, comprising:

(1) measuring concentration of a preselected electrochemically detectable marker, (Marker)WB, naturally present in a preselected volume of whole blood having an initial ratio of red cell volume to plasma volume, the concentration of marker naturally present within red cells being different from its concentration in plasma;

(2) decreasing the ratio of red cell volume to plasma volume by lysing red cells in the preselected volume of whole blood;

(3) measuring the marker concentration after lysing, (Marker)WB; and

(4) calculating the hematocrit using an equation comprising the term;

##EQU4## where;

Hct is hematocrit;

(Marker)WB is measured marker concentration after Lysis;

(Marker)WB is measured marker concentration before Lysis.

2. The method of claim 1 wherein the electrochemically detectable marker is an ion.

3. The method of claim 2 wherein the ion is selected from the group consisting of sodium ion, potassium ion, trimethylphenyl ammonium ion and tetrapentyl ammonium ion.

4. The method of claim 3 wherein the ion is sodium ion.