Non-mechanical buffer circulation apparatus for electrophoresis

• US 4,702,814 A
• Filed: 09/15/1986
• Issued: 10/27/1987
• Est. Priority Date: 09/15/1986
• Status: Expired due to Term

• Current Assignee: Center For Blood Research Incorporated
• Original Assignee: Center For Blood Research Incorporated
• Inventors: Audeh, Zuheir L.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Hsing, Ben
• Application Number:
  US06/907,405
• Time in Patent Office: 407 Days
• Field of Search: 204/275, 204/277, 204/278, 204/299 R, 204/301, 204/302, 204/306, 204/180.1
• US Class Current: 204/616
• CPC Class Codes: G01N 27/44704 Details; Accessories

Abstract

An improvement in electrophoretic apparatus is provided for non-mechanical fluid transfer between the anode and cathode electrode containing chambers of the apparatus. Buffering fluid is transferred from one electrode containing chamber to the other via gas collecting means and a conduit which extends at a positive angle of include between the electrode containing chambers. By providing a constant and uniform rate of fluid transfer, a uniform pH value is maintained throughout the apparatus without use of mechanical pumps for the duration of the electrophoresis.

Claims

1. In a electrophoresis apparatus including a housing having at least two chambers and a support block to support a sample during electrophoresis, an anode electrode contained in one chamber and a cathode electrode contained in another chamber, and fluid in each electrode containing chamber sufficient to submerge the support block and the sample during electrophoresis, the improvement comprising:

gas collections means for collecting gas bubbles generated at an electrode; and

a conduit in fluid communication with said gas collection means, said conduit extending at a positive incline from the interior of one electrode containing chamber into the interior of the other electrode containing chamber of fluid transfer between said chambers.

2. The improved electrophoresis apparatus as recited in claim 1 comprising a plurality of said gas collection means and conduits in combination.