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High refractive index haloalkyl-functional shell-core polymers and their use in light scattering immunoassays

  • US 4,703,018 A
  • Filed: 02/20/1985
  • Issued: 10/27/1987
  • Est. Priority Date: 02/20/1985
  • Status: Expired due to Term
First Claim
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1. A particle reagent having high refractive index consisting essentially of:

  • (A) a polymer particle having an inner core and an outer shell wherein the inner core is a polymer having a refractive index of not less than 1.54 as measured at the wavelength of the sodium D line and wherein the outer shell is a polymer of(1) five to about seventy parts by weight of the outer shell of an ethylenically unsaturated monomer having a haloalkyl functional group capable of reacting with a compound of biological interest, its antigen or its antibody, selected from the group consisting of ##STR2## wherein X is Cl or Br and R is H, CH3, or C2 H5, (2) 95 to about 30 parts by weight of other ethylenically unsaturated monomers selected in amounts not to result in the formation of water soluble polymer particles, and(3) not more than 10 parts by weight of the outer shell of the residual monomers of the inner core;

    said outer shell being formed by polymerization in the presence of said inner core; and

    wherein said polymer particle has an approximate diameter range of 0.01-1.0 μ

    m, a 5-100% surface coverage by a monomolecular layer of anionic surfactant, and is covalently attached to(B) a compound of biological interest, its antigen or its antibody.

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