Assay cartridge

• US 4,704,255 A
• Filed: 07/15/1983
• Issued: 11/03/1987
• Est. Priority Date: 07/15/1983
• Status: Expired due to Term

• Current Assignee: Abbott Laboratories
• Original Assignee: Pandex Laboratories, Inc.
• Inventors: Jolley, Michael E.
• Primary Examiner(s): Lacey, David L.
• Assistant Examiner(s): Gzybowski, Michael S.
• Application Number:
  US06/514,170
• Time in Patent Office: 1,572 Days
• Field of Search: 206/509, 210/321.1, 210/416.1, 210/433.2, 210/456, 210/541, 422/101, 422/102, 422/103, 436/177
• US Class Current: 422/534
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

An assay cartridge which has a substantially rectangular base plate, a substantially rectangular top plate, and four side walls. The top plate has a plurality of aligned adjacent reaction wells located on its top side. Each well has a hole at its bottom which opens into a waste reservoir which is the space inside of the base plate, top plate and four walls. A filter membrane is positioned against the underside of the top plate covering the well holes. A port through the base plate allows reducing the pressure in the waste reservoir relative to the pressure over the wells for drawing the liquid phase of a reaction from the well through the filter and into the waste reservoir.

Claims

1. A fully disposable assay cartridge for conducting a pluralilty of solid phase fluorescence immunoassays on materials in which such assays are conducted within said cartridge itself and during the application of reduced pressure in a waste reservoir, said cartridge comprising:

a substantially rectangular base plate;

a substantially rectangular top plate having a top side and an underside, the top plate located opposite to and substantially parallel to the phase, the top plate having a plurality of aligned adjacent wells located on its top side, each well having a top opening with an edge intersecting said top side and a bottom hole at its bottom which extends to the underside of the top plate, said bottom hole of said well being smaller than said top opening, said wells further having lower walls having an inverted frustum shape with a tapered portion extending downwardly and inwardly toward said bottom hole and substantially terminating at, and defining, said bottom hole for concentrating material on which an assay is to be conducted at said bottom hole, and relative top opening, bottom hole and depth dimensions for conduction a solid phase fluorescence immunoassay in said wells including said bottom hole having a diameter no greater than one-half the diameter of said top opening and said depth dimension being sufficiently small relative to said top opening to permit excitation light to clear the edge of said top opening and excite the material concentrated at said bottom hole and to permit emission light from the material concentrated at said bottom hole to clear the edge of said top opening so that the same can be measured;

an intermediate section comprising rear, front and first and second walls having substantially rectangular cross sections and being serially and permanently joined to one another in sealed relationship, said intermediate section positioned between and permantly joined to said base plate and said top plate in sealed relationship to define a cavity between said base and top plates and said intermediate section;

a sealed waste reservoir located beneath the wells of the top plate and inside the cavity defined by the permanently joined base plate, top plate and intermediate section;

a non-removable filter membrane permanently affixed to the underside of the top plate to which said well holes extend, said membrane extending across said bottom hole and being permanently affixed in sealing relationship to the underside of said top plate in an area surrounding said bottom hole whereby an effective filter area for each well is defined by the size of said bottom hole; and

means for facilitating a reduction of pressure in the waste reservoir relative to the pressure over the wells while retaining any waste products in the waste reservoir comprising a port in said waste reservoir, said means facilitating the simultaneous reduction of pressure in said waste reservoir and for conducting a solid phase immunoassay.

2. The assay cartridge of claim 1 wherein the port further comprises a tube which extends into the waste reservoir.

3. The assay cartridge of claim 1 wherein the filter membrane has a pore size of about 10 microns of less.

4. The assay cartridge of claim 1 wherein the base plate, top plate, and intermediate section are molded plastic.

5. The assay cartridge of claim 4 wherein the plastic is acrylic, polystryrene, or polycarbonate.

6. The assay cartridge of claim 5 wherein the filter membrane is cellulose acetate, nitrocellulose, polyvinylidene fluoride, polyvinyl chloride, teflon, polysugone, polyester, polycarbonate, paper or glass fiber.

7. The assay cartridge of claim 1 wherein

the base plate further comprises rear, front and first and second flat base plate lateral surfaces,

the top plate further comprises rear, front and first and second flat top plate lateral surfaces,

the rear, front and first and second walls further comprise respectively rear, front and first and second flat wall lateral surfaces,

the rear base plate lateral surface, the rear top plate lateral surface, and the rear wall lateral surface being contiguous and substantially parallel,

the front base plate lateral surface, the front top plate lateral surface, and the front wall lateral surface being contiguous and substantially parallel,

the first base plate lateral surface, the first top plate lateral surface, and the first wall lateral surface being contiguous and substantially parallel, and

the second base plate lateral surface, the second top plate lateral surface, and the second wall lateral surface being contiguous and substantially parallel.

8. The assay cartridge of claim 7 wherein

the rear wall lateral surface is laterally recessed relative to the rear base plate lateral surface and the rear top plate lateral surface,

the front wall lateral surface is laterally recessed relative to the front base plate lateral surface and the front top plate lateral surface,

the first wall lateral surface is laterally recessed relative to the first base plate lateral surface and the first top plate lateral surface, and

the second wall lateral surface is laterally recessed relative to the second base plate lateral surface and the second top plate lateral surface.

9. The assay cartridge of claim 8 wherein the assay cartridge further comprises rear and front centering pegs, said rear and front centering pegs extending laterally outward respectively from the rear and front wall lateral surfaces and being substantially opposed to one another.

10. The assay cartridge of claim 7 wherein

the base plate further comprises first and second flat base plate corner surfaces, the first base plate corner surface located between the first base plate lateral surface and the front base plate lateral surface, the second base plate corner surface located between the second base plate lateral surface and the front base plate lateral surface, and

the top plate further comprises first and second top plate corner surfaces, the first top plate corner surface located between the first top plate lateral surface and the front top plate lateral surface, the second top plate corner surface located between the second top plate lateral surface and the front top plate lateral surface.

11. The assay cartridge of claim 10 wherein

the top plate further comprises a top plate raised ridge along the upper periphery of the front, rear, first and second top plate lateral surfaces and the first and second top plate corner surfaces,

the base plate further comprises a base plate raised ridge along the lower periphery of the front, rear, first and second base plate lateral surfaces and the first and second base plate corner surfaces, and

the top plate raised ridge and the base plate raised ridge having substantially similar configurations and one raised ridge having slightly smaller dimensions than the other raised ridge.

12. The assay cartridge of claim 11 wherein the base plate further comprises a base plate underside and a channel located along the outer periphery of the base plate underside and between the base plate underside and the base plate ridge.

13. A fully disposable assay cartridge for conducting a plurality of solid phase fluorescenc immunoassays on materials in which such assays are conducted within said cartridge itself and during the application of reduced pressure in a waste reservoir, said cartridge comprising:

a top plate having a top side and a bottom side;

a plurality of assay wells disposed in said top plate, each of said wells extending through said top plate and having a top opening with an edge intersecting said top side, a bottom opening in said bottom side and a side wall portion having the shape of an inverted frustum and extending toward said bottom opening and substantially terminating at and defining said bottom opening, said bottom opening being smaller than said top opening and said side wall portion including a tapered portion tapering inwardly toward said bottom opening to intersect with said bottom opening for concentrating material on which an assay is to be conducted at said bottom opening, said wells having relative top opening, bottom opening and depth dimensions for conducting a plurality of said phase fluorescence immunoassays in said wells including said bottom opening having a diameter no greater than one-half the diameter of said top opening and said depth dimension being sufficiently small relative to said top opening to permit excitation light to clear the edge of said top opening and excite the material concentrated at said bottom opening and to permit emission light from the material concentrated at said bottom opening to clear the edge of said top opening so that the same can be measured;

a non-removable membrane extending across said bottom opening and being permanently affixed in sealing relationship to the bottom side of said top plate in an area surrounding the bottom opening of each of said wells whereby an effective filter area for each well is defined by the size of said bottom opening;

a base plate permanently connected with said top plate in sealed relationship so as to form a sealed waste reservoir between said base plate and the bottom side of said top plate; and

a port in said waste reservoir for facilitating a reduction of pressure in said waste reservoir relative to the pressure in said wells, said port facilitating the simultaneous reduction of pressure in said waste reservoir and for conducting a solid phase immunoasay.

14. The assay cartridge of claim 13 including a raised ridge surrounding the top opening of each of said wells.

15. The assay cartridge of claim 13 wherein the diameter of said bottom opening is less than one-third the diameter of said top opening.

16. The assay cartridge of claim 13 wherein said tapered portion extends inwardly to said bottom opening.

17. The assay cartridge of claim 13 wherein said waste reservoir is permanently sealed.

18. The assay cartridge of claim 13 wherein the side wall portion of each of said wells includes a cylindrical portion adjacent to said top opening and said tapered portion extends between said cylindrical portion and said bottom opening.

19. The assay cartridge of claim 18 wherein said tapered portion forms an acute angle with respect to longitudinal exis of said well.

20. The assay cartridge of claim 13 wherein said top opening has a diameter greater than the depth of said wells.

21. The assay cartridge of claim 20 wherein said top opening is approximately 6.9 mm in diameter, said bottom opening is approximately 2 mm in diamter and the depth of each of said wells is approximately 4.25 mm.

22. A fully disposable assay cartridge for conducting a plurality of solid phase fluorescence immunoassays on materials in which such assays are conducted within said cartridge itself and during the application of reduced pressure in a waste reservoir, said cartridge comprising:

a top plate having a top side and a bottom side;

a plurality of assay wells disposed in said top plate, each of said wells extending through said top plate and having a top opening with an edge intersecting said top side, a bottom opening in said bottom side and a side wall extending between said top and bottom openings comprising a lower tapered side wall portion having the shape of an inverted frustum and substantially terminating at and defining said bottom opening, said bottom opening being smaller than said top opening and said side wall including a tapered portion which tapers inwardly toward said bottom opening for concentrating material on which an assay is to be conducted at said bottom opening, said wells having relative top opening, bottom opening and depth dimensions for conducting a plurality of solid phase fluorescence immunoassays in said wells;

a single sheet of filter membrane permanently fixed to the bottom side of said top plate in an area surrounding the bottom opening of each of said wells so as to preclude the passage of fluid through said bottom opening except through said filter membrane, said membrane extending across said bottom openings and being permanently affixed in sealing relationship to the underside of said bottom openings in the area surrounding said bottom openings whereby an effective filter area for each well is defined by the size of said bottom opening;

a base plate permanently connected with said top plate in sealed relationship so as to form a sealed waste reservoir between said base plate and the bottom side of said top plate; and

a port in said waste reservoir for facilitating a reduction of pressure in said waste reservoir relative to the pressure in said wells, said port facilitating the simultaneous reduction of pressure in said waste reservoir and for conducting a solid phase immunoassay.

23. The assay cartridge of claim 22 wherein said filter membrane is joined to the bottom side of said top plate in an area surrounding the bottom opening of each of said wells.

24. The assay cartridge of claim 23 wherein said filter membrane is bonded to the bottom side of said top plate and maintained in such position solely by said bonding.

25. The assay cartridge of claim 23 wherein said filter membrane is joined with the underside of said top plate by molding the underside of said top plate directly to said filter membrane.

26. The assay cartridge of claim 25 wherein said filter membrane is maintained in position against the bottom side of said top plate solely as a result of said molding.