Nucleic acid hybridization assay and detectable molecules useful in such assay
First Claim
1. A detectable molecule of the formula
whereA3 is an oligo- or polynucleotide having at least one modifiable reactive group consisting of amino, hydroxy, cis OH, halide, aryl, imidazoyl, carbonyl, carboxyl, thiol or a residue comprising an activated carbon;
--X-- is selected from the group consisting of ##STR54## --R1 -- is ##STR55## a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH;
--Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where --R2 -- is a C1 -C10 branched or unbranched alkyl;
Za is chlorine, bromine or iodine;
--E-- is O, NH or an acyclic divalent sulfur atom;
Detb is a chemical moiety capable of being detected, comprising biotin or a substituted or unsubstituted metal chelator or a compound capable of yielding a metal chelator; and
m is an integer from 1 to the total number of modified reactive groups on said oligo- or polynucleotide.
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Accused Products
Abstract
A detectable molecule of the formula
A.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.m
where A3 is A2 or a polymer, where A3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## --R1 -- is ##STR2## or a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where R2 is a C1 -C10 branched or unbranched alkyl; Za is chlorine, bromine or iodine; E is O, NH or an acyclic divalent sulfur atom; Detb is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR3## or the 4-hydroxy or acyloxy derivative thereof, where R3 is C1 -C4 alkyl or CH2 COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C1 -C10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A3. The detectable molecules are useful in in virto or in vivo assays or therapy.
244 Citations
28 Claims
- 1. A detectable molecule of the formula
- space="preserve" listing-type="equation">A.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.m
where A3 is an oligo- or polynucleotide having at least one modifiable reactive group consisting of amino, hydroxy, cis OH, halide, aryl, imidazoyl, carbonyl, carboxyl, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR54## --R1 -- is ##STR55## a C1 -C10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R2 -- where --R2 -- is a C1 -C10 branched or unbranched alkyl; Za is chlorine, bromine or iodine; --E-- is O, NH or an acyclic divalent sulfur atom; Detb is a chemical moiety capable of being detected, comprising biotin or a substituted or unsubstituted metal chelator or a compound capable of yielding a metal chelator; and m is an integer from 1 to the total number of modified reactive groups on said oligo- or polynucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
Specification
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- Resources
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Current AssigneeEnzo Diagnostics, Inc.
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Original AssigneeEnzo Biochem Incorporated
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InventorsStavrianopoulos, Jannis G.
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Primary Examiner(s)Nucker, Christine M.
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Application NumberUS06/575,396Time in Patent Office1,387 DaysField of Search536/26, 536/27, 435/6, 935/78US Class Current435/6.14CPC Class CodesA61K 51/0497 conjugates with a carrier b...A61K 51/1093 conjugates with carriers be...G01N 33/533 with fluorescent labelG01N 33/534 with radioactive labelG01N 33/553 Metal or metal coated
