Method and apparatus for clinical analysis

• US 4,708,940 A
• Filed: 03/10/1983
• Issued: 11/24/1987
• Est. Priority Date: 03/10/1982
• Status: Expired due to Fees

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Ono, Naoya, Yoshida, Kasumi, Takano, Nobuyoshi
• Primary Examiner(s): Marcus, Michael S.
• Application Number:
  US06/473,867
• Time in Patent Office: 1,720 Days
• Field of Search: 422/72, 422/64, 422/67, 422/73, 422/100, 436/45, 494/16, 494/17, 494/42, 494/56, 494/57
• US Class Current: 436/45
• CPC Class Codes: B04B 2011/046 Loading, unloading, manipulating sample containers
• CPC Class Codes: B04B 5/0421 pivotably mounted
• CPC Class Codes: G01N 2035/00495 Centrifuges
• CPC Class Codes: G01N 2035/00504 combined with carousels
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 2035/1086 Cylindrical, e.g. variable angle
• CPC Class Codes: G01N 35/0095 introducing urgent samples with priority, e.g. Short Turn Around Time Samples [STATS]
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor

Abstract

A clinical analyzer with an analyzing section having a reaction tube and photometer, and a sampling section having a sample transfer pipe and centrifugal separator which also serves as a sampler. Sample containers containing blood are set on a plurality of container holders through an aperture formed in the cover of the centrifugal separator. A rotor with the container holders mounted thereon is rotated at a low speed intermittently so that each of the container holders is positioned at the container setting position in a predetermined order in which a container holder which is not located adjacently to the previously positioned container holder is positioned next.

Subsequently, the rotor is rotated at a high speed for a predetermined period so that the sample liquid in each sample container centrifugally separated. After the centrifugal separation, the rotor is rotated at a low speed intermittently so that the sample containers set on the container holders are positioned to the sample take-out position in the same order as in setting the sample containers. A pipe is inserted through a sample transfer hole formed in the cover of the centrifugal separator into the sample container, and the sample liquid is pumped up and transported to the analyzing section. This series of operations is repeated and a plurality of samples are measured for items of analysis by the photometer sequentially.

Claims

1. A method for clinical analysis comprising the steps of:

(a) rotating a rotor intermittently at a low speed to adjust each of a plurality of container holders connected to said rotor at a first position and inserting a sample container containing whole blood therein in one of said container holders;

(b) rotating said rotor at a high speed so as to direct the bottom of each of said sample containers outward centrifugally to separate the whole blood contained in said sample containers;

(c) rotating said rotor intermittently at a low speed to adjust repetitively each of said sample containers at a second position for taking-out a portion of the separated whole blood;

(d) inserting a liquid take-out pipe into a layer in the separated whole blood in each of said sample containers to take-out a sample portion of the separated whole blood while said sample container held in said container holder is placed at said take-out position; and

(e) measuring the sample introduced into the analyzing section through said liquid take-out pipe in accordance with an item of analysis.

2. A method for clinical analysis according to claim 1 comprising a step of introducing a plurality of analytical items into said liquid take-out pipe while each of said sample containers stays at said sample take-out position.

3. A method for clinical analysis according to claim 1 comprising a step of positioning said container holders sequentially to said first position after sample liquids have been taken out by said liquid take-out pipe, and lifting each positioned sample container.

4. A method for clinical analysis according to claim 1, comprising the steps of introducing a reagent into a flow path while a sample liquid is transported in said flow path, and introducing a reactive liquid into a flow cell within a photometer.

5. A method for clinical analysis according to claim 1, wherein said sample containers are kept substantially horizontal during said high speed rotation and substantially vertical during said low speed rotation.

6. A clinical analyzer comprising:

a sampling section having a sample liquid take-out means and a centrifugal separation means, said centrifugal separation means having a rotor provided with a plurality of container holders in which sample containers can be held, said centrifugal separation means having a drive means for successively rotating said rotor intermittently so that each container holder stops temporarily at a container setting position such that a sample container can be fed into a sample holder, rotating said rotor continuously at a high speed during a centrifugal separating operation of the sample within a sample container, and moving said rotor intermittently at a low speed so that each container holder stops temporarily at a sample take-out position during a sample take-out operation,

an analyzing section which measures an introduced liquid sample in accordance with an item of analysis,

means defining a flow path between the sampling section and the analyzing section, wherein

said sample liquid take-out means operates to take out sample liquid in a container holder positioned at said sample take-out position and to introduce said liquid toward said analyzing section, and said analyzing section comprises means for mixing a sample liquid introduced through the take-out means with a reagent inside the flow path, and a photometer for measuring a mixed liquid.

7. A clinical analyzer according to claim 6, comprising a flow cell in the analyzing section, and means for introducing a sample for a plurality of different items into the flow cell in doses at spaced intervals.

8. A clinical analyzer according to claim 6, further comprising:

means for lifting said container holder when the sample container on said container holder is in a position to remove the sample container after taking-out a sample therefrom.

9. A clinical analyzer comprising an analyzing section which measures an introduced liquid sample in accordance with an item of analysis;

and a sampling section having a sample liquid take-out means and means for centrifugal separation, said centrifugal separation means having a rotor provided with a plurality of container holders in which sample containers can be set, said centrifugal separation means having a drive means for moving said rotor intermittently so that each of said container holder stops at a container setting position during a sample setting operation, for rotating said rotor at a high speed during a centrifugal separation operation, and for moving said rotor intermittently so that each of said container holders stops temporarily at a sample take-out position during a sample take-out operation, said centrifugal separation means having a holder position detector which detects the position of each container holder and halts the operation of said rotor, said sample liquid take-out means operating to take out sample liquid in a container holder positioned at said sample take-out position and introduce said liquid toward said analyzing section.

10. A clinical analyzer according to claim 9, wherein said sample take-out pipe has an associated liquid level sensing electrode.

11. A clinical analizer according to claim 9 comprising a means for lifting a sample container set in a container holder when said container holder is positioned at a container take-out position.

12. A clinical analyzer according to claim 9, wherein means is provided to keep container holders in which sample containers are set in a slant attitude when said rotor rotates at high speed.

13. A clinical analyzer according to claim 9, wherein said drive means is provided for positioning, subsequently to the positioning for a first container holder, a second container holder located at a position symmetrically opposite to a position of said first container holder with respect to the center of rotation to said container setting position.

14. A clinical analyzer according to claim 9, wherein said centrifugal separation means has a means for covering said rotor, said cover means being provided therein with a first aperture at said container setting position for transferring a sample container, and a second aperture at said sample take-out position for inserting a sample take-out pipe.

15. A clinical analyzer according to claim 14 comprising a lid means which is open when a sample container is set on said container holder and closes said first aperture when said rotor rotates at a high speed.

16. A method for clinical analysis wherein a rotor having therein a plurality of container holders is kept at a predetermined position comprising:

(a) a first step of rotating said rotor intermittently to position each of a plurality of empty container holders at an inserting position so as to insert each of a plurality of said sample containers containing whole blood therein,

(b) a second step of rotating said rotor at a high speed to centrifugally separate said whole blood contained in said sample containers into blood clot and blood serum,

(c) a third step of rotating said rotor to position said container holders each holding one of said sample containers including the blood clot and the blood serum at an aspirating position so as to aspirate the blood serum from said sample containers, and

(d) a fourth step of rotating said rotor intermittently to position each of said container holders at said inserting position for taking to said sample containers including the blood clot from said container holders, wherein the blood serum aspirated in said third step is led through a take-out pipe inserted into said respective sample containers into a flow cell of an analyzing section.

17. A method for clinical analysis according to claim 16, wherein the positioning operation of said third step and the positioning operation of said fourth step are synchronized.

18. A method for clinical analysis according to claim 16, wherein reagent is added to the blood serum in a flow conduit provided between said take-out pipe and said flow cell.

19. A method for clinical analysis wherein a rotor having therein a plurality of container holders is kept at a predetermined position, comprising:

(a) a first step of rotating said rotor intermittently to position each of a plurality of empty container holders at an inserting position so as to insert each of said sample containers containing whole blood therein,

(b) a second step of rotating said rotor at a high speed to centrifugally separate said whole blood contained in said sample containers into blood clot and blood serum,

(c) a third step of rotating said rotor to position said container holders each holding one of said sample containers including the blood clot and the blood serum at an aspirating position so as to aspirate the blood serum from said sample containers, and

(d) a fourth step of rotating said rotor intermittently to position each of said container holders at said inserting position for taking out said sample containers including the blood clot from said container holders, wherein each of said sample containers are rotated to said inserting position so as to be inserted in turn in each of said container holders in an order for forming a pair thereof located in a substantially opposite direction with respect to the center of said rotor, and the blood serum aspirated in said third step is led through a take-out pipe inserted into said respective sample containers into a flow cell of an analyzing section.

20. A clinical analyzer comprising:

(a) a rotor means having a rotor and a plurality of container holders arranged on said rotor,

(b) means for intermittently rotating said rotor in a first mode to successively position each of a plurality of empty container holders at a single inserting position so as to insert a sample container containing whole blood therein into each of said container holders so as to be located in turn in a substantially opposite position with respect to the center of said rotor,

(c) means for inserting a take-out pipe into said sample container held in said container holder located at said aspirating position in said third mode so as to aspirate blood serum separated from said whole blood in said sample container into said take-out pipe,

(d) cover means having a container aperture for inserting therethrough said sample container at said inserting position and a pipe aperture for inserting therethrough said take-out pipe for covering said rotor, and

(e) flow cell means for measuring said blood serum which has flowed through said take-out pipe.

21. A clinical analyzer according to claim 20, further comprising:

flow conduit means for connecting said take-out pipe with said flow cell, and

reagent means for leading reagent through a switching valve into said flow conduit means.

22. A clinical analyzer according to claim 20, further comprising:

lid means for closing said container aperture, and

means for activating said lid means to close said container aperture in said second mode and to open said container aperture in said first and fourth modes.

23. A clinical analyzer comprising:

(a) rotor means having a rotor and a plurality of container holders arranged on said rotor and provided in an analyzer for conducting a first mode intermittently rotating said rotor to successively position each of a plurality of empty container holders at a single inserting position so as to insert each of said sample containers containing whole blood therein into each of said sample containers, a second mode rotating said rotor at a high speed to centrifugally separate said whole blood contained in said sample containers, a third mode rotating said rotor intermittently to position said container holders at an aspirating position, and a fourth mode rotating said rotor intermittently to position each of said container holders at said inserting position for taking out said sample containers,

(b) means for inserting a take-out pipe into said sample container held in said container holder located at said aspirating position in said third mode so as to aspirate blood serum separated from said whole blood in said sample container into said take-up pipe,

(c) cover means having a container aperture of inserting therethrough said sample container at said inserting position and a pipe aperture of inserting therethrough said take-out pipe for covering said rotor,

(d) means for lifting said sample container held in said container holder indexed in said inserting position so as to project an upper portion of said lifted sample container over said container aperture of said cover means, and

(e) an analyzing means for measuring said blood serum which has flowed through said take-out pipe.