Sensor for components of a liquid mixture

• US 4,711,245 A
• Filed: 05/07/1984
• Issued: 12/08/1987
• Est. Priority Date: 05/05/1983
• Status: Expired due to Term

• Current Assignee: Medisense Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Generics International Incorporated (Endo International PLC)
• Inventors: Higgins, Irving J., Hill, Hugh A. O., Plotkin, Elliot V.
• Primary Examiner(s): Cohen, Lee S.
• Application Number:
  US06/607,699
• Time in Patent Office: 1,310 Days
• Field of Search: 128/635, 204/403, 204/415
• US Class Current: 204/403.1
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: A61B 5/6848 Needles
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

This invention relates to equipment and methods for detecting the presence of, measuring the amount of, and/or monitoring the level of, one or more selected components in a liquid mixture, employing an electrode sensing system.

We have discovered that a class of mediating compounds has extremely useful properties for mediating the transfer of charge between enzyme-catalyzed reactions and electrode surfaces (15) in electrode sensing systems.

Specifically, the specification discloses as electrode sensor mediators, organometallic compounds composed of at least two organic rings, each of which has at least two double bonds in a conjugated relationship; a metal atom is in electron-sharing contact with those rings. An enzyme capable of catalyzing a reaction at a rate representative of the selected compound concentration is in contact with an assay mixture, and the mediator compound transfers charge between the enzyme and the conductive surface of the electrode at a rate representative of the enzyme catalyzed reaction rate.

Claims

1. An electrode sensor system for sensing the presence of at least one selected component of a mixture of components, said sensor system comprising:

(a) at least two electrically conducting means insulated from each other, each said conducting means having an electrically conductive surface for transferring electrical signals, each said surface being adapted to be in electrical contact with said mixture,

(b) an enzyme, the catalytic activity of said enzyme being indicative of the presence of said component,

(c) a mediator compound which transfers electrons between said enzyme and one said conductive surface when said enzyme is catalytically active, said mediator being an organometallic compound which comprises at least two organic rings, each of which is characterised by at least two double bonds that are conjugated and a metal atom in electron-sharing contact with each of said rings, and

(d) means for measuring current resulting from said electron transfer at said one conductive surface, said measuring means being electrically connected to each of said electrically conducting means.

2. The system of claim 1 wherein said mediator is confined at said one electrically conductive surface.

3. The system of claim 1 wherein said enzyme is confined at said one electrically conductive surface.

4. The system of claim 1, said enzyme being further characterized in that it catalyzes a reaction of said selected component.

5. The system of claim 1 wherein said mediator compound is a ferrocene.

6. The system of claim 5 wherein said ferrocene is selected from the group consisting of ferrocene;

1,1'"'"'-dicarboxyferrocene;

carboxyferrocene;

vinylferrocene;

1,1'"'"'dimethylferrocene;

ferrocene monocarboxylic acid;

hydroxyethylferrocene; and

1,1'"'"'bis-hydroxymethyl ferrocene.

7. The system as claimed in claim 5 in which the enzyme is a glucose oxidase.

8. The sensor system of claim 7 for use in a liquid mixture including glucose, to be responsive to the presence of glucose, one said conducting means comprising an electrode composed of carbon, and a layer of said ferrocene at an external surface thereof as an electron-transferring mediator compound, and said glucose oxidase being located upon the layer of mediator compound.

9. A sensor system as claimed in claim 8 in which the ferrocene is deposited on said one surface from a readily evaporable organic solvent therefor.

10. A sensor system as claimed in claim 8 in which the enzyme is a glucose oxidase immobilised on the mediator by DCC.

11. A sensor system as claimed in claim 8 having a protective membrane permeable to water and glucose molecules, said membrane being positioned around the one said electrically conductive surface.

12. The sensor system of claim 11 in which the protective membrane is a layer of cellulose acetate deposited from a solution thereof.

13. The sensor system of claim 1 or claim 7 wherein said mediator compound is freely diffusing.

14. The system of claim 1 wherein said enzyme is a non-oxygen-specific flavo-protein.

15. The system of claim 14 wherein said flavo-protein is selected from the group consisting of aldehyde oxidase, pyruvate oxidase, xanthine oxidase, and glucose oxidase.

16. The system of claim 1 wherein said enzyme is a quino-protein.

17. The system of claim 16 wherein said quino-protein is selected from the group consisting of glucose dehydrogenase, alcohol dehydrogenase and methanol dehydrogenase.

18. The system of claim 1 wherein said metal atom is iron.

19. The system as claimed in claim 1 in which the enzyme catalyses a reaction of glucose whereby there is provided a glucose sensor.

20. A sensor system as claimed in claim 19 in which the enzyme is a bacterial glucose dehydrogenase.

21. A sensor system as claimed in claim 20 in which the glucose dehydrogenase is that separated from Acinetobacter calcoaceticus.

22. A sensor system as claimed in claim 1 in which at least one said electrically conducting means is made of a material chosen from silver, carbon particle paste and solid carbon.

23. A sensor system as claimed in claim 1 in which the enzyme is a bacterial glucose dehydrogenase held in a gelatine layer at the surface of the mediator layer.

24. The sensor system of claim 1 comprising means for implantation of said electrically conducting means in a human subject.

25. A sensor system of claim 1 wherein said enzyme is characterized by the ability to catalyze a reaction resulting in transfer of electrons to said enzyme, whereby said mediator compound transfers electrons from said enzyme to said conductive surface.

26. The system of claim 1 in which the enzyme is in haem-containing enzyme.

27. The system of claim 27 in which the haem-containing enzyme is chosen from the group consisting of yeast cytochrome c peroxidase and horseradish peroxidase.