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Modified nucleotides and methods of preparing and using same

DC CAFC
  • US 4,711,955 A
  • Filed: 05/23/1983
  • Issued: 12/08/1987
  • Est. Priority Date: 04/17/1981
  • Status: Expired due to Term
First Claim
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1. A nucleotide or oligo- or polynucleotide sequence comprising at least one of a moiety having the structure:

  • wherein B represents a 7-deazapurine or a pyrimidine moiety;

    wherein A represents a moiety selected from the group consisting of biotin and iminobiotin;

    provided that if B is a 7-deazapurine, A is attached to the 7-position of the deazapurine, and if B is a pyrimidine, A is attached to the 5-position of the pyrimidine, A being attached to B directly or through a linkage group, said linkage group not interfering substantially with the characteristic ability of A to form a detectable complex with one of avidin, streptavidin or antibodies to biotin or iminobiotin.

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