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Determination of total creatine kinase or an isoenzyme with a multilayer analytical element

  • US 4,713,327 A
  • Filed: 05/01/1985
  • Issued: 12/15/1987
  • Est. Priority Date: 05/01/1985
  • Status: Expired due to Fees
First Claim
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1. A multilayer analytical element for the determination of total creatine kinase or a creatine kinase isoenyzme, said element comprising three or more layers and containing in one or more of said layers, a substrate for creatine kinase, said element also comprising a support having thereon,a registration layer containing a binder material at a coverage of at least about 8 g/m2 and an indicator composition which provides a detectable change in response to the reaction of said substrate with said creatine kinase or said isoenzyme,a reagent layer, andan isotropically porous spreading layer containing an activator for creatine kinase, said layers being in order from said support and in fluid contact.

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